Recombinant Human Active Chk2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1358-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Chk2 protein
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N terminal GST tag
SDS-PAGE
88 kDa
Activity
The specific activity of Chk2 was determined to be 660 nmol/min/mg using a synthetic peptide substrate (KKKVSRSGLYRSPSMPENLNRPR).
Scientific Data Images for Recombinant Human Active Chk2 Protein, CF
Recombinant Human Active Chk2 Protein SDS-PAGE.
The approximate molecular weight is 88 kDa and the purity is > 90%.Formulation, Preparation and Storage
1358-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: Chk2
Chk2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage where the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogates both phosphorylation of p53 on Serine 20 and p53 stabilization (2).
References
- Matsuoka, S. et al. (1998) Science 282:1893.
- Chehab, N.H. et al. (2000) Genes Dev. 14:278.
Long Name
Checkpoint Kinase 2
Alternate Names
CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53
Gene Symbol
CHEK2
UniProt
Additional Chk2 Products
Product Documents for Recombinant Human Active Chk2 Protein, CF
Product Specific Notices for Recombinant Human Active Chk2 Protein, CF
For research use only
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