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Recombinant Human Active Coagulation Factor XIV/ProteinC, CF

R&D Systems, part of Bio-Techne | Catalog # 4998-SE

R&D Systems, part of Bio-Techne
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4998-SE-010

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Coagulation Factor XIV/Protein C protein
Ala43-Pro461, with a C-terminal 10-His tag
The isolated protein was activated by thrombin/thrombomodulin complex and further purified.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala43 (light chain) & Leu212 (heavy chain)

Predicted Molecular Mass

19 kDa (light chain), 29 kDa (heavy chain)

SDS-PAGE

22-25 kDa and 40-50 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).
The specific activity is >275 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

4998-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Coagulation Factor XIV/Protein C

Protein C (PROC) is a vitamin K-dependent serine protease synthesized in liver as a single-chain precursor (1). The N-terminus consists of a signal peptide (aa 1-32) and a propeptide (aa 33-42). The mature chain (aa 43-461) is converted to two disulfide-linked chains (light: aa 43-199 and heavy: 200‑461) and both forms are inactive. The light chain consists of Gla (gamma-carboxy-glutamate) domain and two EGF-like domains. The heavy chain consists of an activation peptide (aa 200‑211) and serine protease domain (aa 212-450). Present in plasma at 3 to 5 mg/L, PROC plays a key role in anticoagulation. Physiologically, the inactive forms of PROC are converted to the active form by thrombin, which releases the activation peptide. The active PROC cleaves factors VIIIa and Va to inactivate them. This anticoagulation activity can be enhanced by a presence of a cofactor such as Protein S. In hereditary thrombophilia, PROC deficiency is caused by a genetic mutation which affects PROC activity. A severe recessive form may result in massive thrombosis fatal to patient.

References

  1. Shen, L. and Dahlbäck, B. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1673.

Long Name

Vitamin K-dependent Protein C

Alternate Names

Autoprothrombin IIA, PROC, Protein C

Entrez Gene IDs

5624 (Human); 19123 (Mouse); 25268 (Rat)

Gene Symbol

PROC

UniProt

Additional Coagulation Factor XIV/Protein C Products

Product Documents for Recombinant Human Active Coagulation Factor XIV/ProteinC, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active Coagulation Factor XIV/ProteinC, CF

For research use only

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