Recombinant Human Active Coagulation Factor XIV/ProteinC, CF
R&D Systems, part of Bio-Techne | Catalog # 4998-SE
Key Product Details
Source
Accession #
Structure / Form
Conjugate
Applications
Product Specifications
Source
Ala43-Pro461, with a C-terminal 10-His tag
The isolated protein was activated by thrombin/thrombomodulin complex and further purified.
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The specific activity is >275 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4998-SE
Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Coagulation Factor XIV/Protein C
Protein C (PROC) is a vitamin K-dependent serine protease synthesized in liver as a single-chain precursor (1). The N-terminus consists of a signal peptide (aa 1-32) and a propeptide (aa 33-42). The mature chain (aa 43-461) is converted to two disulfide-linked chains (light: aa 43-199 and heavy: 200‑461) and both forms are inactive. The light chain consists of Gla (gamma-carboxy-glutamate) domain and two EGF-like domains. The heavy chain consists of an activation peptide (aa 200‑211) and serine protease domain (aa 212-450). Present in plasma at 3 to 5 mg/L, PROC plays a key role in anticoagulation. Physiologically, the inactive forms of PROC are converted to the active form by thrombin, which releases the activation peptide. The active PROC cleaves factors VIIIa and Va to inactivate them. This anticoagulation activity can be enhanced by a presence of a cofactor such as Protein S. In hereditary thrombophilia, PROC deficiency is caused by a genetic mutation which affects PROC activity. A severe recessive form may result in massive thrombosis fatal to patient.
References
- Shen, L. and Dahlbäck, B. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1673.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Coagulation Factor XIV/Protein C Products
Product Documents for Recombinant Human Active Coagulation Factor XIV/ProteinC, CF
Product Specific Notices for Recombinant Human Active Coagulation Factor XIV/ProteinC, CF
For research use only