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Recombinant Human Active FAK Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4467-KS

R&D Systems, part of Bio-Techne
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4467-KS-010

Key Product Details

Source

Sf 9 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human FAK protein

Purity

>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N-terminal His tag

SDS-PAGE

35 kDa

Activity

The specific activity of FAK was determined to be 51 nmol/min/mg as per Activity Assay Protocol, and was equivalent to 291.5 nmol/min/mg as per radiometric assay.

Scientific Data Images for Recombinant Human Active FAK Protein, CF

Recombinant Human Active FAK Protein SDS-PAGE

Recombinant Human Active FAK Protein SDS-PAGE.

The approximate molecular weight is 35 kDa and the purity is > 75%.

Formulation, Preparation and Storage

4467-KS
Formulation Supplied in 50 mM Sodium Phosphate (pH 7.0), 300 mM NaCl, 150 mM Imidazole, 0.1 mM PMSF, 0.25 mM DTT, and 25% Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: FAK

FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation (1). Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness (2). Elevated FAK expression in anaplastic astrocytoma and glioblastoma tumor biopsy samples has been demonstrated.

References

  1. Schaller, M.D. (2001) Biochim. Biophys. Acta 1540:1.
  2. Gabarra-Niecko, V. et al. (2003) Cancer Metastasis Rev. 22:359.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Entrez Gene IDs

5747 (Human); 14083 (Mouse); 25614 (Rat)

Gene Symbol

PTK2

UniProt

Additional FAK Products

Product Documents for Recombinant Human Active FAK Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active FAK Protein, CF

For research use only

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