Recombinant Human Active GSK-3 beta Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2506-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human GSK-3 beta protein
Purity
>70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
73 kDa
Activity
The specific activity of GSK-3 beta was determined to be 224 nmol/min/mg using a synthetic peptide substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE).
Reviewed Applications
Read 1 review rated 5 using 2506-KS in the following applications:
Scientific Data Images for Recombinant Human Active GSK-3 beta Protein, CF
Recombinant Human Active GSK-3 beta Protein SDS-PAGE.
The approximate molecular weight is 73 kDa and the purity is > 70%.Formulation, Preparation and Storage
2506-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: GSK-3 beta
GSK-3 beta is a Serine/Threonine protein kinase that was originally identified as the kinase that phosphorylates and inhibits glycogen synthase (1). GSK-3 beta is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin and modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK-3 beta into Chinese hamster ovary cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau at all the sites investigated with phosphorylation-dependent anti-tau antibodies (2).
References
- Sutherland, C. et al. (1993) Biochem. J. 296:15.
- Sperber, B.R. et al. (1995) Neurosci. Lett. 197:149.
Long Name
Glycogen Synthase Kinase 3 beta
Alternate Names
GSK3 beta, GSK3B
Gene Symbol
GSK3B
UniProt
Additional GSK-3 beta Products
Product Documents for Recombinant Human Active GSK-3 beta Protein, CF
Product Specific Notices for Recombinant Human Active GSK-3 beta Protein, CF
For research use only
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