Recombinant Human Active PKC delta Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4585-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human PKC delta protein
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
104 kDa
Activity
The specific activity of PKC delta was determined to be 185 nmol/min/mg using a synthetic peptide substrate.
Scientific Data Images for Recombinant Human Active PKC delta Protein, CF
Recombinant Human Active PKC delta Protein SDS-PAGE.
The approximate molecular weight is 104 kDa and the purity is > 90%.Formulation, Preparation and Storage
4585-KS
| Formulation | Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 10 mM Glutathione, 0.1 mM EDTA, 0.1 mM PMSF, and 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ -70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: PKC delta
Protein Kinase C delta (PKC delta) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 104 kDa protein kinase that shows strict dependence on the presence of phospholipids but shows no activation by Ca2+ (1). Phosphatidylinositol is the most potent activator of PKC delta. Northern blot analysis indicates that PKC delta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells (2). PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters.
References
- Leibersperger, H. et al. (1991) J. Biol. Chem. 266:14778.
- Mischak, H. et al. (1991) Biochemistry 30:7925.
Long Name
Protein Kinase C delta
Alternate Names
PRKCD
Entrez Gene IDs
5580 (Human)
Gene Symbol
PRKCD
UniProt
Additional PKC delta Products
Product Documents for Recombinant Human Active PKC delta Protein, CF
Product Specific Notices for Recombinant Human Active PKC delta Protein, CF
For research use only
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