Recombinant Human Active ROCK1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4590-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human ROCK1 protein
aa 17-535
aa 17-535
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
85 kDa
Activity
The specific activity of ROCK1 is typically 49-67 nmol/min/mg using a synthetic peptide substrate.
Scientific Data Images for Recombinant Human Active ROCK1 Protein, CF
Recombinant Human Active ROCK1 Protein SDS-PAGE.
The approximate molecular weight is 85 kDa and the purity is > 80%.Formulation, Preparation and Storage
4590-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: ROCK1
ROCK1 is a ubiquitously expressed serine/threonine kinase that is a downstream target of the small GTPase RhoA. ROCK1 is involved in diverse cellular functions, including smooth muscle contraction, actin cytoskeleton organization, cell adhesion and motility, and gene expression (1). ROCK1 contributes to the development of cardiac fibrosis and induction of fibrogenic cytokines in cardiomyocytes in response to pathological stimuli. ROCK1 knockout mice exhibit reduced perivascular and interstitial fibrosis, which is associated with reduced expression of a variety of extracellular matrix (ECM) proteins and fibrogenic cytokines (2).
References
- Zhao, Y.M. et al. (2004) Dev. Biol. 275:183.
- Zhang, C. et al. (2006) FASEB J. 20:916.
Long Name
Rho-associated, Coiled-Coil Containing Protein Kinase 1
Alternate Names
p160ROCK, ROK beta
Gene Symbol
ROCK1
UniProt
Additional ROCK1 Products
Product Documents for Recombinant Human Active ROCK1 Protein, CF
Product Specific Notices for Recombinant Human Active ROCK1 Protein, CF
For research use only
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