Recombinant Human Active ZAP70 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3709-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human ZAP70 protein
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
96 kDa
Activity
The specific activity of ZAP70 was determined to be 13 nmol/min/mg using a Poly (Glu:Tyr, 4:1) synthetic peptide substrate.
Scientific Data Images for Recombinant Human Active ZAP70 Protein, CF
Recombinant Human Active ZAP70 Protein SDS-PAGE.
The approximate molecular weight is 96 kDa and the purity is > 90%.Formulation, Preparation and Storage
3709-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: ZAP70
ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/ZAP70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes that contain ZAP70 (1). TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex (2).
References
- Duplay, P. et al. (1994) J. Exp. Med. 179:1163.
- Chan, A.C. et al. (1994) J. Immunol. 152:4758.
Long Name
Zeta-chain [TCR] Associated Protein Kinase 70 kDa
Alternate Names
mrtle, mur, SRK, STD, TZK
Gene Symbol
ZAP70
UniProt
Additional ZAP70 Products
Product Documents for Recombinant Human Active ZAP70 Protein, CF
Product Specific Notices for Recombinant Human Active ZAP70 Protein, CF
For research use only
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