Recombinant Human Activin RIIA Fc Chimera (CHO) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 340-RC2

R&D Systems, part of Bio-Techne
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340-RC2-100
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Key Product Details

Source

CHO

Accession #

P27037

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Activin RIIA Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Activin RIIA protein
Human Activin RIIA
(Met1 - Pro134)
Accession # P27037		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala20

Predicted Molecular Mass

40 kDa (monomer)

SDS-PAGE

60-67 kDa, reducing conditions

Activity

Measured by its ability to inhibit Activin A-induced hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340.

Approximately 0.01-0.05 μg/mL of Recombinant Human (rh) Activin RIIA Fc Chimera will inhibit 50% of the biological response due to 3 ng/mL of rhActivin A.

Formulation, Preparation and Storage

340-RC2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIIA

Activin proteins are involved in a wide range of biological processes including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, the regulation of reproductive physiology, inflammation, and carcinogenesis (1 ‑ 3). They function through heteromeric complexes of type I and type II serine/threonine kinase receptors (2, 4). Dimeric ligands bind to a type II receptor, such as Activin Receptor IIA (ActRIIA), which then associates with a type I receptor to initiate signal transduction (4). ActRIIA mediates the pleiotropic effects of Activins and Inhibins as well as several members of the BMP and GDF families of TGF-beta like proteins (4). Mature human ActRIIA is a 70 kDa glycoprotein that consists of a 116 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 352 aa cytoplasmic region that includes the kinase domain and a PDZ-binding motif (5). Within the ECD, human ActRIIA shares 98% aa sequence identity with mouse and rat ActRIIA. Signaling through ActRIIA is modulated by its interaction with RGM-B/DRAGON, Cripto, Endoglin/CD105, TGF‑ beta RIII/Betaglycan, or BAMBI (6 - 10). These interactions can enhance ligand‑induced signaling or interfere with signaling by preventing ActRIIA association with type I receptors (6 - 9). Activin‑induced responses can also be limited by the enhanced internalization of ActRIIA following its association with the cytoplasmic protein ARIP2 (11).

References

  1. Welt, C. et al. (2002) Exp. Biol. Med. 227:724.
  2. Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
  3. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
  4. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  5. Matzuk, M.M. and A. Bradley (1992) Biochim. Biophys Acta 1130:105.
  6. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
  7. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
  8. Lewis, K.A. et al. (2000) Nature 404:411.
  9. Onichtchouk, D. et al. (1999) Nature 401:480.
  10. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  11. Matsuzaki, T. et al. (2002) J. Biol. Chem. 277:19008.

Long Name

Activin Receptor IIA

Alternate Names

ActivinRIIA, ACVR2A, AVR2A

Entrez Gene IDs

92 (Human); 11480 (Mouse)

Gene Symbol

ACVR2A

UniProt

P27037

Additional Activin RIIA Products

Product Documents for Recombinant Human Activin RIIA Fc Chimera (CHO) Protein, CF

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Product Specific Notices for Recombinant Human Activin RIIA Fc Chimera (CHO) Protein, CF

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