Recombinant Human ADAM23 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4974-AD

R&D Systems, part of Bio-Techne
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4974-AD-050
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Key Product Details

Source

CHO

Accession #

O75077

Conjugate

Unconjugated

Applications

Bioactivity

View all ADAM23 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ADAM23 protein
Ser60-His585 with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser60 (pro) & Ala287 (mature)

Predicted Molecular Mass

60.8 kDa (pro) & 34.9 kDa (mature)

SDS-PAGE

40‑60 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.
The ED50 for this effect is 1.0‑4.0 μg/mL.

Formulation, Preparation and Storage

4974-AD
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM23

ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 100 kDa glycosylated proprotein that consists of a 227 amino acid (aa) propeptide, a 506 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 19 aa cytoplasmic domain (1, 2). The ECD contains a nonfunctional metalloprotease domain, an integrin‑binding disintegrin domain, and a cysteine‑rich region. Proteolytic removal of the propeptide generates an approximately 70 kDa mature protein (2). Within the propeptide, peptidase, and disintegrin domains (aa 60‑585), human ADAM23 shares 93% aa sequence identity with mouse and rat ADAM23. Alternative splicing of human ADAM23 yields additional isoforms (including a potentially secreted molecule) by substitution of the transmembrane and cytoplasmic regions (3). ADAM23 is expressed in the brain, notably on pyramidal cells of the cerebral cortex and hippocampus and on cerebellar Purkinje and granule cells (1, 2, 4‑6). ADAM23 binds the prion protein PrPC and the leucine‑rich glioma inactivated proteins LGI1 and LGI4 (7, 8). Its interaction with LGI1 promotes neurite outgrowth and dendrite arborization on pyramidal neurons (5). LGI1 appears to function as a trans‑synaptic bridge between ADAM23 and ADAM22 (6). In adipose tissue, the binding of ADAM23 to LGI3 inhibits adipocyte differentiation and lipid accumulation (9). ADAM23 is also expressed on bronchial epithelial cells (10). It serves as a counter‑receptor for Integrin alphaV beta3 and can limit integrin activation and adhesion to alphaV beta3 ligands (4, 11). ADAM23 expression is down‑regulated in some breast and non‑small cell lung carcinomas (10, 12).

References

  1. Sagane, K. et al. (1998) Biochem. J. 334:93.
  2. Goldsmith, A.P. et al. (2004) J. Neurosci. Res. 78:647.
  3. Sun, Y.P. et al. (2004) Gene 325:171.
  4. Cal, S. et al. (2000) Mol. Biol. Cell. 11:1457.
  5. Owuor, K. et al. (2009) Mol. Cell. Neurosci. 42:448.
  6. Fukata, Y. et al. (2010) Proc. Natl. Acad. Sci. USA 107:3799.
  7. Costa, M.D.M. et al. (2009) Neurosci. Lett. 461:16.
  8. Sagane, K. et al. (2008) Int. J. Biol. Sci. 4:387.
  9. Kim, H.A. et al. (2012) Biochim. Biophys. Acta 1821:914.
  10. Hu, C. et al. (2011) Int. J. Exp. Pathol. 92:333.
  11. Verbisck, N.V. et al. (2009) Cancer Res. 69:5546.
  12. Costa, F.F. et al. (2004) Oncogene 23:1481.

Long Name

A Disintegrin and Metalloproteinase Domain 23

Alternate Names

MDC-L, MDC3

Entrez Gene IDs

8745 (Human); 23792 (Mouse); 301460 (Rat)

Gene Symbol

ADAM23

UniProt

O75077

Additional ADAM23 Products

Product Documents for Recombinant Human ADAM23 Protein, CF

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Product Specific Notices for Recombinant Human ADAM23 Protein, CF

For research use only

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