Recombinant Human Adenosine Deaminase 2/CECR1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7518-AD

R&D Systems, part of Bio-Techne
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7518-AD-010
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Key Product Details

Source

CHO

Accession #

AAF65941

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Adenosine Deaminase 2/CECR1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Adenosine Deaminase 2/CECR1 protein
Ile30-Lys511, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile30

Predicted Molecular Mass

57 kDa

SDS-PAGE

58-66 kDa, reducing conditions

Activity

Measured by the ability to catalyze the hydrolytic deamination of adenosine to inosine.
The specific activity is >14,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7518-AD
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Adenosine Deaminase 2/CECR1

Adenosine deaminase is one of the key enzymes of purine nucleotide metabolism. It catalyses the conversion of adenosine and deoxy-adenosine to inosine and deoxy-inosine, respectively (1, 2). Adenosine Deaminase 2 (ADA2) is also known as CECR1 because it is a candidate gene for cat eye syndrome, a developmental disorder (3). ADA is a secreted protein that is expressed in many tissues, with the highest expression in lymphoblasts, heart, lung, and placenta (4). ADA2 is a member of a family of adenosine deaminase-related growth factors (ADGFs), proteins that are involved in tissue development (4). ADA2 induces the differentiation of monocytes into macrophages and stimulates the proliferation of T helper cells and macrophages by a mechanism independent of its catalytic activity (5). It has been suggested that ADA2 could be a therapeutic target for the control of immune responses in inflammation and cancer (5).

References

  1. Wolfenden, R.V. et al. (1969) Biochemistry 6:2412.
  2. Lowenstein, J.M. (1972) Physiol. Rev. 52:382.
  3. Riazi, M.A. et al. (2000) Genomics 64:277.
  4. Zavialov, A.V. and A. Engstrom (2005) Biochem. J. 391:51.
  5. Zavialov, A.V. et al. (2010) J. Leucoc. Biol. 88:279.

Long Name

Adenosine Aminohydrolase

Alternate Names

ADA2, ADGF, CECR1, IDGFL

Entrez Gene IDs

51816 (Human)

Gene Symbol

ADA2

UniProt

AAF65941

Additional Adenosine Deaminase 2/CECR1 Products

Product Documents for Recombinant Human Adenosine Deaminase 2/CECR1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Adenosine Deaminase 2/CECR1 Protein, CF

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