Recombinant Human ALCAM/CD166 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11191-AL

R&D Systems, part of Bio-Techne
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11191-AL-100
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Key Product Details

Source

CHO

Accession #

Q13740.2

Conjugate

Unconjugated

Applications

Bioactivity

View all ALCAM/CD166 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ALCAM/CD166 protein
Trp28-Ala526, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp28

Predicted Molecular Mass

57 kDa

SDS-PAGE

65-85 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human ALCAM/CD166 His-tag (Catalog # 11191-AL) is immobilized at 0.5 μg/mL (100 µL/well), Recombinant Human CD6 Fc Chimera (Catalog # 627-CD) binds with an ED50 of 30.0-240 ng/mL.

Scientific Data Images for Recombinant Human ALCAM/CD166 His-tag Protein, CF

Recombinant Human ALCAM/CD166 His-tag Protein Binding Activity.

When Recombinant Human ALCAM/CD166 His-tag Protein (Catalog # 11191-AL) is immobilized at 0.5 μg/mL (100 µL/well), Recombinant Human CD6 Fc Chimera (627-CD) binds with an ED50 of 30.0-240 ng/mL.

Recombinant Human ALCAM/CD166 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human ALCAM/CD166 His-tag Protein (Catalog # 11191-AL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-85 kDa.

Formulation, Preparation and Storage

11191-AL
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ALCAM/CD166

Activated leukocyte cell adhesion molecule (ALCAM), also known as CD166, is a surface glycoprotein belonging to the immunoglobulin superfamily (1, 2). ALCAM, along with MCAM and BCAM/Lu, form a small subgroup of adhesion molecules involved in tissue development and maintenance, neurogenesis, and regulation of immune responses (3). Mature human ALCAM consists of an extracellular domain (ECD) containing 2 IgV and 3 IgC domains, a transmembrane domain, and a short cytoplasmic domain (1, 2). Within the ECD, human ALCAM shares 93% amino acid sequence identity with both mouse and rat ALCAM. An isoform of ALCAM with a shorter stalk region in the ECD is known to exist and is associated with higher shedding tendency and decreased cell adhesion (2). Initially found expressed on activated leukocytes, ALCAM expression has been detected in a wide variety of tissues and cells (3, 4). In addition to cell adhesion, other ALCAM functions include leukocyte migration across the blood brain barrier, T-cell activation, and osteogenesis, (2, 5, 6). Overexpression of ALCAM is often associated with poor prognosis in various human tumors, such as bladder cancer, prostate cancer, melanoma, and liver cancer (2,7).

References

  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Ferragut, F. et al. (2021) Cytokine Growth Factor Rev. 61:27.
  3. Swart, G.W. (2002) Eur. J. Cell Biol. 81:313.
  4. Zimmerman, A.W. et al. (2006) Blood 107:3212.
  5. Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
  6. Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
  7. Darvishi, B. et al. (2020) Exp. & Mol. Path 115:104443.

Long Name

Activated Leukocyte Cell Adhesion Molecule

Alternate Names

CD166

Entrez Gene IDs

214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)

Gene Symbol

ALCAM

UniProt

Q13740.2

Additional ALCAM/CD166 Products

Product Documents for Recombinant Human ALCAM/CD166 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human ALCAM/CD166 His-tag Protein, CF

For research use only

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