Recombinant Human ALK/CD246 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4210-CD

R&D Systems, part of Bio-Techne
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4210-CD-100
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Key Product Details

Source

NS0

Accession #

Q9UM73.3

Conjugate

Unconjugated

Applications

Bioactivity

View all ALK/CD246 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ALK/CD246 protein
Val19-Ser1038, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val19

Predicted Molecular Mass

111 kDa

SDS-PAGE

130-150 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
In a Human ALK/CD246 Antibody (Catalog # AF4210) coated plate, when Recombinant Human ALK/CD246 is present at 0.5 μg/mL, Recombinant Human Pleiotrophin/PTN (Catalog # 252-PL) binds with an ED50 of 20-100 ng/mL.

Scientific Data Images for Recombinant Human ALK/CD246 His-tag Protein, CF

In a Human ALK/CD246 Antibody (AF4210) coated plate, when Recombinant Human ALK/CD246 (Catalog # 4210-CD) is present at 0.5 μg/mL, Recombinant Human Pleiotrophin/PTN (252-PL) binds with an ED50 of 20-100 ng/mL.
2 μg/lane of Recombinant Human ALK.CD246 His-tag (Catalog # 4210-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-150 kDa.

Formulation, Preparation and Storage

4210-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK/CD246

Anaplastic Lymphoma Kinase (ALK), also known as CD246, is a transmembrane receptor tyrosine kinase in the Insulin Receptor family. The ALK gene is a target of multiple chromosomal translocations in cancer that encode hybrid proteins which promote cellular transformation (1, 2). Human ALK consists of a 1020 amino acid (aa) extracellular domain (ECD) with two MAM domains that flank an LDLR class A domain, a 21 aa transmembrane segment, and a 561 aa cytoplasmic domain that contains the tyrosine kinase domain (3, 4). Within the ECD, human ALK shares 89% aa sequence identity with mouse and rat ALK. It is primarily expressed in the developing nervous system but is also found in various non‑neural tissues (3 ‑ 6). Mature ALK is expressed on the cell surface as a 200 ‑ 220 kDa N‑glycosylated protein (3, 4, 7). Proteolytic cleavage of ALK liberates an 80 kDa soluble fragment from the ECD with a 140 kDa fragment remaining cell‑associated (8, 9). ALK is classified as a dependence receptor, a protein that promotes apoptosis in the absence of ligand but is anti‑apoptotic upon stimulation (2, 7). Its cytoplasmic domain is cleaved following Asp1160 by Caspases during apoptosis (7). ALK stimulation by antibody ligation induces activation of its kinase domain and receptor phosphorylation, enabling the association of ALK with signal transduction proteins (4, 9). ALK binds the cytokines Pleiotrophin and Midkine, although their effects on cellular responses are not consistent between different systems (8, 10 ‑ 12). ALK promotes neurite formation in neuroblastoma cells and mediates the neuroprotective effects of Pleiotrophin in motor neurons (9, 12). A t(2;5) chromosomal translocation with Nucleophosmin (NPM) in human anaplastic large cell lymphoma results in a hybrid protein consisting of NPM fused to the kinase domain of ALK (6, 13). NPM‑ALK as well as other ALK fusion proteins are aberrantly localized to the cytoplasm and nucleus, have constitutively active kinase domains, and promote cellular transformation (1, 13, 14).

References

  1. Chiarle, R. et al. (2008) Nat. Rev. Cancer 8:11.
  2. Allouche, M. (2007) Cell Cycle 6:1533.
  3. Iwahara, T. et al. (1997) Oncogene 14:439.
  4. Morris, S.W. et al. (1997) Oncogene 14:2175.
  5. Vernersson, E. et al. (2006) Gene Expr. Patterns 6:448.
  6. Morris, S.W. et al. (1994) Science 263:1281.
  7. Mourali, J. et al. (2006) Mol. Cell. Biol. 26:6209.
  8. Moog-Lutz, C. et al. (2005) J. Biol. Chem. 280:26039.
  9. Motegi, A. et al. (2004) J. Cell Sci. 117:3319.
  10. Stoica, G.E. et al. (2001) J. Biol. Chem. 276:16772.
  11. Stoica, G.E. et al. (2002) J. Biol. Chem. 277:35990.
  12. Mi, R. et al. (2007) Proc. Natl. Acad. Sci. 104:4664.
  13. Fujimoto, J. et al. (1996) Proc. Natl. Acad. Sci. 93:4181.
  14. Bischof, D. et al. (1997) Mol. Cell. Biol. 17:2312.

Long Name

Anaplastic Lymphoma Kinase

Alternate Names

CD246, Ki-1, Tcrz

Entrez Gene IDs

238 (Human); 11682 (Mouse); 266802 (Rat)

Gene Symbol

ALK

UniProt

Q9UM73.3

Additional ALK/CD246 Products

Product Documents for Recombinant Human ALK/CD246 His-tag Protein, CF

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