Recombinant Human Alkaline Phosphatase/ALPL Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2909-AP
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Alkaline Phosphatase/ALPL protein
Leu18-Ser502, with a C-terminal 6-His tag
Leu18-Ser502, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu18
Predicted Molecular Mass
54 kDa
SDS-PAGE
65-75 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).
The specific activity is >30,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >30,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2909-AP
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, MgCl2 and ZnCl2. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Alkaline Phosphatase/ALPL
Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue‑nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503 to 524) is not present in the mature form.
References
- Le Du, M.-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
- Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
- Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Long Name
Alkaline Phosphatase Liver
Alternate Names
Akp2, AP-TNAP, HOPS, TNAP, TNSALP
Gene Symbol
ALPL
UniProt
Additional Alkaline Phosphatase/ALPL Products
Product Documents for Recombinant Human Alkaline Phosphatase/ALPL Protein, CF
Product Specific Notices for Recombinant Human Alkaline Phosphatase/ALPL Protein, CF
For research use only
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