Recombinant Human alpha 1B-Glycoprotein Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7757-GP

R&D Systems, part of Bio-Techne
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7757-GP-050
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Key Product Details

Source

HEK293

Accession #

EAW72575

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

View all alpha 1B-Glycoprotein Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human alpha 1B-Glycoprotein protein
Ala22-Ser495, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Starts at Ala22

Predicted Molecular Mass

52.8 kDa

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CRISP‑3 (Catalog # 2397-CR) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human  alpha 1B‑Glycoprotein that produces 50% of the optimal binding response is approximately 0.4‑2.4 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 7757-GP in the following applications:

Formulation, Preparation and Storage

7757-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 1B-Glycoprotein

Alpha-1B-glycoprotein (A1BG) is an approximately 80 kDa molecule that circulates in the serum (1). Mature human A1BG consists of five tandem Ig‑like domains and shares 44% amino acid sequence identity with mouse and rat A1BG (2, 3). Alternative splicing generates an isoform that lacks the signal peptide and the first Ig‑like domain. A1BG binds with high affinity to the cysteine‑rich protein CRISP‑3 (4). In pancreatic carcinoma, it is elevated in pancreatic juice as well as in the serum relative to normal patients (5, 6). A1BG is abnormally excreted into the urine during idiopathic nephrotic syndrome (INS) (7). Multiple breakdown fragments of A1BG are also present in INS urine, including one of 14 kDa that is found in steroid‑resistant but not steroid‑sensitive cases (7). A1BG is also elevated in the urine of patients with interstitial cystitis/painful bladder syndrome (IC/PBS) (8).

References

  1. Schultze, H.E. et al. (1963) Nature 200:1103.
  2. Ishioka, N. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2363.
  3. Gardmo, C. et al. (2001) Endocrinology 142:2695.
  4. Udby, L. et al. (2004) Biochemistry 43:12877.
  5. Tian, M. et al. (2008) BMC Cancer 8:241.
  6. Li, C. et al. (2009) J. Proteome Res. 8:483.
  7. Piyaphanee, N. et al. (2011) Proteomics Clin. Appl. 5:334.
  8. Goo, Y.A. et al. (2010) Int. Braz. J. Urol. 36:464.

Alternate Names

A1B, A1BG, ABG, alpha 1BGlycoprotein, GAB

Entrez Gene IDs

1 (Human); 117586 (Mouse)

Gene Symbol

A1BG

UniProt

EAW72575

Additional alpha 1B-Glycoprotein Products

Product Documents for Recombinant Human alpha 1B-Glycoprotein Protein, CF

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Product Specific Notices for Recombinant Human alpha 1B-Glycoprotein Protein, CF

For research use only

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