Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7836-AM

R&D Systems, part of Bio-Techne
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7836-AM-050
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Key Product Details

Source

HEK293

Accession #

NP_653271

Conjugate

Unconjugated

Applications

Bioactivity

View all alpha 2-Macroglobulin-like 1/A2ML1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human alpha 2-Macroglobulin-like 1/A2ML1 protein
Glu18-Glu1454, with a C-terminal 6-His tag

Purity

>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Starts at Glu18

Predicted Molecular Mass

160 kDa

SDS-PAGE

155‑175 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human alpha2‑Macroglobulin-like1 is immobilized at 2 μg/mL (100 µL/well), the concentration of Recombinant Human LRP‑1 Cluster II Fc Chimera (Catalog # 2368-L2) that produces 50% of the optimal binding response is approximately 1‑5 μg/mL.

Formulation, Preparation and Storage

7836-AM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 2-Macroglobulin-like 1/A2ML1

alpha2-Macroglobulin like-1 (A2ML1) is an approximately 180 kDa secreted glycoprotein that is structurally and functionally similar to alpha2‑Macroglobulin (A2M) (1). Mature human A2ML1 shares 40% amino acid sequence identity with human A2M. It functions as a broad spectrum protease inhibitor with activity toward chymotrypsin, papain, thermolysin, and subtilisin A (2). Like A2M, A2ML1 binds to proteases covalently and contains a bait region (2). When the bait region is cleaved by a targeted protease, A2M undergoes a conformational change which blocks the protease’s active site and also promotes A2M clearance from the circulation (1). In contrast to the dimeric and tetrameric A2M, A2ML1 is secreted as a monomer (2, 3). A2ML1 is expressed in epidermis, testis, thymus, and placenta (2). It binds to Kallikrein 7, a protease that regulates epidermal desquamation by disrupting corneocyte adhesion (2, 4). The C‑terminal domain of A2ML1 mediates binding to LRP1 on the surface of macrophages followed by endocytosis (5). A2M has a similar receptor‑binding domain which is responsible for A2M interactions with LRP1 (6). A2ML1 is also known as the keratinocyte p170 autoantigen in the skin disease paraneoplastic pemphigus (7). During zebrafish development, an A2ML1-like protein is required for liver formation (8).

References

  1. Sottrup-Jensen, L. (1989) J. Biol. Chem. 264:11539.
  2. Galliano, M.-F. et al. (2006) J. Biol. Chem. 281:5780.
  3. Gettins, P.G. et al. (1995) J. Biol. Chem. 270:14160.
  4. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
  5. Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
  6. Sottrup-Jensen, L. et al. (1986) FEBS Lett. 205:20.
  7. Schepens, I. et al. (2010) PLoS ONE 5:e12250.
  8. Hong, S.-K. and I.B. Dawid (2008) PLoS ONE 3:e3736.

Alternate Names

A2ML1, alpha 2Macroglobulin like 1, CPAMD9

Entrez Gene IDs

144568 (Human)

Gene Symbol

A2ML1

UniProt

NP_653271 (Human)

Additional alpha 2-Macroglobulin-like 1/A2ML1 Products

Product Documents for Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF

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Product Specific Notices for Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF

For research use only

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