Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF
R&D Systems, part of Bio-Techne | Catalog # AVI11180
His-tag Avi-tag
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human alpha-L-Iduronidase/IDUA protein
Human IDUA (Ala26-Pro653) Accession # P35475.2 |
6-His tag | Avi-tag |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala26
Predicted Molecular Mass
73 kDa
SDS-PAGE
81-91 kDa, under reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human alpha‑L‑Iduronidase/IDUA His-tag Avi-tag binds to Human alpha-L-Iduronidase/IDUA Antibody (Catalog # AF4119) with an ED50 of 0.800-8.00 ng/mL.
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl alpha-L-iduronide.
The specific activity is >10,000 pmol/min/μg, as measured under the described conditions.
Biotinylated Recombinant Human alpha‑L‑Iduronidase/IDUA His-tag Avi-tag binds to Human alpha-L-Iduronidase/IDUA Antibody (Catalog # AF4119) with an ED50 of 0.800-8.00 ng/mL.
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl alpha-L-iduronide.
The specific activity is >10,000 pmol/min/μg, as measured under the described conditions.
Scientific Data Images for Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF
Biotinylated Recombinant Human alpha‑L‑Iduronidase/IDUA His-tag Avi-tag Protein Binding Activity.
Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human alpha-L-Iduronidase/IDUA His-tag Avi-tag binds (Catalog # AVI11180) to Human alpha -L-Iduronidase/IDUA Antibody (AF4119) with an ED50 of 0.800-8.00 ng/mL.Biotinylated Recombinant Human alpha‑L‑Iduronidase/IDUA His-tag Avi-tag Protein Enzyme Activity.
Biotinylated Recombinant Human alpha‑L‑Iduronidase/IDUA His-tag Avi-tag Protein (Catalog # AVI11180) is measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl alpha-L-iduronide.Biotinylated Recombinant Human alpha‑L‑Iduronidase/IDUA His-tag Avi-tag Protein SDS-PAGE.
2 μg/lane of Biotinylated Recombinant Human alpha‑L‑Iduronidase/IDUA His-tag Avi-tag Protein (Catalog # AVI11180) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 81-91 kDa, under reducing conditions.Formulation, Preparation and Storage
AVI11180
Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: alpha-L-Iduronidase/IDUA
References
- Maita, N. et al. (2013) Proc. Natl. Acad. Sci. 110:14628.
- Scott, H.S. et al. (1995) Hum. Mutat. 6:288.
- Wraith, J.E. (2005) Expert Opin. Pharmacother. 6:489.
- Jameson, E. (2016) Cochrane Database Syst. Rev. 4: CD009354.
- Kodric, K. et al. (2016) Wien Klin Wochenschr. 128:480.
- Niu, T. et al. (2016) J. Bone Miner. Res. 31:358.
Alternate Names
alphaLIduronidase, IDA, IDUA
Gene Symbol
IDUA
UniProt
Additional alpha-L-Iduronidase/IDUA Products
Product Documents for Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF
Product Specific Notices for Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF
For research use only
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