Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2334-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Aminopeptidase PILS/ARTS1 protein
Ala37-Met941, with a C-terminal 10-His tag
Ala37-Met941, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala37
Predicted Molecular Mass
105 kDa
SDS-PAGE
108 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC.
The specific activity is >125 pmol/min/µg, as measured under the described conditions.
The specific activity is >125 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2334-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Aminopeptidase PILS/ARTS1
The name of Aminopetidase PILS (Puromycin-Insensitive Leucyl-Specific) describes the two basic properties of this zinc metalloprotease in vitro (1). Also known as ALAP (Adipocyte-derived Leucin AminoPeptidase), type 1 tumor necrosis factor receptor (TNFR) shedding aminopeptidase regulator and ER aminopeptidase ERAP1 or ERAAP, it is encoded by the ARTS1 gene (2-4). Aminopeptidase PILS has been identified to regulate antigen presentation, promote TNFR1 ectodomain shedding and associate with hypertension (2-5).
References
- Schomburg, L. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 311, Academic Press, San Diego.
- Cui, X. et al. (2002) J. Clin. Invest. 110:515.
- York, I.A. et al. (2002) Nat. Immunol. 3:1177.
- Serwold, T. et al. (2002) Nature 419:480.
- Yamamoto, N. et al. (2002) Hum. Mutat. 19:251.
Long Name
Aminopeptidase Puromycin-insensitive Leucyl-specific
Alternate Names
A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP
Gene Symbol
ERAP1
UniProt
Additional Aminopeptidase PILS/ARTS1 Products
Product Documents for Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF
Product Specific Notices for Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF
For research use only
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