Recombinant Human APLP-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9605-AL

R&D Systems, part of Bio-Techne
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9605-AL-050
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Key Product Details

Source

NS0

Accession #

P51693-1

Conjugate

Unconjugated

View all APLP-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human APLP-1 protein
Gln34-Glu580, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln34 (blocked)

Predicted Molecular Mass

62 kDa

SDS-PAGE

74-89 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9605-AL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APLP-1

Amyloid Precursor-like Proteins (APLPs) are members of the amyloid precursor protein (APP) superfamily of transmembrane proteins. APLP-1, APLP-2, and APP share highly homologous amino acid sequences, except that APLPs lack the beta-amyloid domain derived by proteolytic processing from APP (1). APLP-1 is expressed post-synaptically in cortical neurons (2). APLP-1 is post-translationally modified by N-glycosylation and displays an apparent molecular mass of 80-90 kDa in reducing SDS-PAGE (3). Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by MMPs and secretases, generating multiple fragments from the extracellular and intracellular domains (4). These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator (4). The extracellular domain contains heparin and collagen binding regions. human APLP-1 shares 88% and 86% aa sequence identity with mouse and rat APLP-1, respectively.

References

  1. Suzuki, T. et al. (1997) Biochemistry 36:4643.
  2. Kim, T. W. et al. (1995) Brain Res. Mol. Brain Res. 32:36.
  3. Paliga, K. et al. (1997) Eur. J. Biochem. 250:354.
  4. Scheinfeld, M. H. et al. (2002) J. Biol. Chem. 277:44195.

Long Name

Amyloid-like Protein 1

Alternate Names

APLP1

Entrez Gene IDs

333 (Human); 11803 (Mouse); 502317 (Rat)

Gene Symbol

APLP1

UniProt

P51693-1

Additional APLP-1 Products

Product Documents for Recombinant Human APLP-1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human APLP-1 Protein, CF

For research use only

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