Recombinant Human Apolipoprotein M Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4550-AM

R&D Systems, part of Bio-Techne
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4550-AM-050
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Key Product Details

Source

E. coli

Accession #

NP_061974

Conjugate

Unconjugated

Applications

Bioactivity

View all Apolipoprotein M/ApoM Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Apolipoprotein M/ApoM protein
Cys23-Asn188, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met & Thr62

Predicted Molecular Mass

19.5 kDa

SDS-PAGE

19-21 kDa, reducing conditions

Activity

Measured by its ability to bind all-trans-retinoic acid. The concentration of all-trans-retinoic acid required to quench 50% of Trp fluorescence in Recombinant Human Apolipoprotein M/ApoM is approximately 10-50 μM.

Reviewed Applications

Read 1 review rated 5 using 4550-AM in the following applications:

Formulation, Preparation and Storage

4550-AM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein M/ApoM

Apolipoprotein M (ApoM) is an approximately 25 kDa variably glycosylated protein that adopts a beta-barrel structure characteristic of lipocalin family proteins. It functions as a component of lipoprotein particles which play essential roles in fatty acid and cholesterol transport and metabolism (1, 2). Human ApoM shares 83% amino acid sequence identity with mouse and rat ApoM (3). Alternative splicing generates a short isoform that lacks the N-terminal 72 amino acids. ApoM is produced primarily by hepatocytes but also by renal tubule epithelial cells (4). The signal peptide is not cleaved and is required for ApoM association with lipoprotein particles as well as Megalin mediated reabsorption by the kidney (5, 6). ApoM is cleared from the circulation by the ubiquitously expressed LDL R (7, 8). The majority of circulating ApoM is associated with HDL, while a smaller fraction is associated with LDL (3, 9, 10). It promotes the intracellular formation of large pre-beta (discoidal) HDL particles which inhibit the development of atherosclerotic plaques by facilitating the efflux of cellular cholesterol and preventing phospholipid oxidation (7, 9, 11‑13). The lipocalin structure of ApoM enables it to function as a carrier protein for small hydrophobic molecules including retinol (Vitamin A), all-trans-retinoic acid, 9-cis-retinoic acid, and sphingosine-1-phosphate (S1P) (14, 15). ApoM delivers S1P to S1P1 receptors and contributes to the maintenance of vascular endothelial integrity (15).

References

  1. Christoffersen, C. and L.B. Nielsen (2013) Curr. Opin. Lipidol. May 6 Epub. PMID 23652568.
  2. Arkensteijn, B.W. et al. (2013) Int. J. Mol. Sci. 14:4419.
  3. Xu, N. and B. Dahlback (1999) J. Biol. Chem. 274:31286.
  4. Zhang X.Y. et al. (2003) Acta Histochem. 105:67.
  5. Christoffersen, C. et al. (2008) J. Biol. Chem. 283:18765.
  6. Faber, K. et al. (2006) Mol. Endocrinol. 20:212.
  7. Christoffersen, C. et al. (2010) Circ. Res. 106:1624.
  8. Christoffersen, C. et al. (2012) J. Lipid Res. 53:2198.
  9. Christoffersen, C. et al. (2008) J. Biol. Chem. 283:1839.
  10. Christoffersen, C. et al. (2006) J. Lipid Res. 47:1833.
  11. Mulya, A. et al. (2010) J. Lipid Res. 51:514.
  12. Wolfrum, C. et al. (2005) Nat. Med. 11:418.
  13. Elsoe, S. et al. (2012) Atherosclerosis 221:91.
  14. Ahnstrom, J. et al. (2007) J. Lipid Res. 48:1754.
  15. Christoffersen, C. et al. (2011) Proc. Natl. Acad. Sci. USA 108:9613.

Alternate Names

APOM, NG20, Protein G3a

Entrez Gene IDs

55937 (Human); 55938 (Mouse); 55939 (Rat)

Gene Symbol

APOM

UniProt

NP_061974

Additional Apolipoprotein M/ApoM Products

Product Documents for Recombinant Human Apolipoprotein M Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Apolipoprotein M Protein, CF

For research use only

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