Recombinant Human ASAH2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3557-AH
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human ASAH2/N-acylsphingosine Amidohydrolase-2 protein
Thr15-Ile761, with an N-terminal 6-His tag
Thr15-Ile761, with an N-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
83 kDa
SDS-PAGE
95-140 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid.
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
3557-AH
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl and Sodium Cholate. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ASAH2/N-acylsphingosine Amidohydrolase-2
The human ASAH2 gene encodes N-acylsphingosine amidohydrolase-2, also known as neutral or non-lysosomal ceramidase. ASAH2 is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). An N-terminally truncated form of human ASAH2 has been shown to localize to mitochondria (4). A major physiological function of ASAH2 is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).
References
- Tani, M. et al. (2003) J. Biol. Chem. 278:10523.
- Kono, M. et al. (2006) J. Biol. Chem. 281:7324.
- Mitsutake, S. et al. (2001) J. Biol. Chem. 276:26249.
- El Bawab, S. et al. (2000) J. Biol. Chem. 275:21508.
Long Name
Neutral Ceramidase
Alternate Names
Nacylsphingosine Amidohydrolase2
Gene Symbol
ASAH2
UniProt
Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products
Product Documents for Recombinant Human ASAH2 Protein, CF
Product Specific Notices for Recombinant Human ASAH2 Protein, CF
For research use only
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