Recombinant Human ASGR2/ASGPR2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9370-AS

R&D Systems, part of Bio-Techne
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9370-AS-050
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Key Product Details

Source

CHO

Accession #

NP_001172

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ASGR2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ASGR2 protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human ASGR2/ASGPR2
(Gln80-Ala311)
Accession # NP_001172
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

53 kDa

SDS-PAGE

60-74 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human ASGR2 Fc Chimer binds human plasma von Willebrand Factor with an ED50 of 0.15-0.9 μg/mL.

Scientific Data Images for Recombinant Human ASGR2/ASGPR2 Fc Chimera Protein, CF

Recombinant Human ASGR2/ASGPR2 Fc Chimera Protein Binding Activity

Recombinant Human ASGR2/ASGPR2 Fc Chimera Protein Binding Activity

Immobilized Recombinant Human ASGR2 Fc Chimera binds human plasma von Willebrand Factor with an ED50 of 0.15-0.9 μg/mL.

Formulation, Preparation and Storage

9370-AS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: ASGR2

Asialoglycoprotein receptor 2 (ASGR-2), also known as C-type lectin domain family 4 member H2 (CLEC4H2) and hepatic lectin H2 (HL-2), is an approximately 48 kDa member of the C-type lectin receptor family (1, 2). ASGR-2 is a single-pass type II transmembrane protein that plays a role in the endocytosis of desialylated glycoproteins and hepatic thrombopoietin production (3). ASGR-2 is closely related to ASGR-1 which is about 2 kDa smaller and is encoded by distinct but closely linked genes. Variations in ASGR-2 structure due to alternative splicing have been reported (4-6). Mature ASGR-2 consists of a 5 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment and a 232 aa extracellular domain (ECD). Within the ECD, human ASGR-2 shares 66% and 62% aa sequence identity with mouse and rat ASGR-2, respectively. Both ASGR-2 and ASGR-1 compose the asialoglycoprotein receptor (ASGPR) which mediates removal of potentially hazardous glycoconjugates from blood in health and disease (7). ASGPR, also known as the Ashwell receptor, can modulate von Willebrand factor (vWF) and platelet homeostasis in part through clearance of platelets that are first desialylated during sepsis caused by pathogens including S. pneumoniae. The ASGR-1 chain of the Ashwell receptor can also participate in plasma vWF clearance independently of sialylation and sepsis, ASGR-2 colocalization with plasma vWF was increased in ASGR-1 deficient mice (8). In addition, ASGR-2 expression was shown to be associated with malignant phenotypes in gastric cancer and may represent a specific biomarker for recurrence of some gastric cancers (9).

References

  1. Spiess, M. and H.F. Lodish. (1985) Proc Natl Acad Sci U.S.A. 19: 6465.
  2. Grewal, P.K. (2010) Methods Enzymol. 479:223.
  3. Grozovsky, R. et al. (2015) Nat Med. 1:47.
  4. Drickamer, K. et al. (1984) J. Biol. Chem. 259:770.
  5. Halberg, D.F. et al. (1987) J. Biol. Chem. 262:9828.
  6. Paietta, E. et al. (1992) J. Biol. Chem. 267:11078.
  7. Harris R.L. et al. (2012) Mol Biol Int. 2012:283974.
  8. Grewal, P. et al. (2008) Nature Medicine. 14:6.
  9. Tanaka H. et al. (2018) Mol Cancer Res. doi: 10.1158/1541. MCR-17-0467. [Epub ahead of print].

Long Name

Asialoglycoprotein Receptor 2

Alternate Names

ASGPR2, CLEC4H2, HBXBP

Entrez Gene IDs

433 (Human); 11890 (Mouse); 29403 (Rat)

Gene Symbol

ASGR2

UniProt

NP_001172

Additional ASGR2 Products

Product Documents for Recombinant Human ASGR2/ASGPR2 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human ASGR2/ASGPR2 Fc Chimera Protein, CF

For research use only

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