Recombinant Human ASP/C3a desArg Protein

R&D Systems, part of Bio-Techne | Catalog # 9620-C3

R&D Systems, part of Bio-Techne
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Carrier Free
9620-C3-025/CF
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With Carrier
9620-C3-025
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Key Product Details

Source

E. coli

Accession #

P01024

Conjugate

Unconjugated

Applications

Bioactivity

View all ASP/C3a desArg Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human ASP/C3a desArg protein
Ser672-Ala747

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser672

Predicted Molecular Mass

9 kDa

SDS-PAGE

9 kDa, reducing conditions

Activity

Measured by its ability to induce IL-6 secretion by LPS-stimulated human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Human ASP/C3a desArg Protein

Recombinant Human ASP/C3a desArg Protein Bioactivity

Recombinant Human ASP/C3a desArg Protein Bioactivity

Recombinant Human ASP/C3a desArg (Catalog # 9620‑C3) induces IL‑6 secretion from LPS stimulated PBMCs. The ED50 for this effect is 0.4‑2.4 μg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9620-C3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 9620-C3/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASP/C3a desArg

Acylation-stimulating protein (ASP), also known as C3adesArg, is an approximately 14 kDa member of the complement family that plays a role in the regulation of triglyceride synthesis (1-4). The secreted protein is a polypeptide fragment comprising amino acids (aa) 672-747 of the Complement C3 precursor protein (4). The polypeptide results from desargination of the carboxyl terminus of C3a, which is generated from interactions among C3, factor B, and adipsin (4). ASP contains a 36 aa anaphylatoxin-like domain (5). Human ASP shares 68% sequence identity with mouse and rat ASP. Increased levels of ASP are usually associated with insulin resistance, obesity and diabetes. It promotes triacylglycerol synthesis in adipocytes and inflammatory cytokine production in a septic environment (6-8).

References

  1. Cianflone, K. et al. (1989) J. Biol. Chem. 264:426.
  2. Baldo, A. et al. (1993) J. Clin. Invest. 92:1543.
  3. Havel, P. (2002) Curr. Opin. Lipidol. 13:51.
  4. Cianflone, K. et al. (2003) Biochim. Biophys. Acta. 1609:127.
  5. Kolev, M. et al. (2017) Front. Immunol. 8:1.
  6. Murray, I. et al. (1999) Biochem. J. 342:41.
  7. Munkonda, M. N. et al. (2012) PLOS One. 7(10):1.
  8. Takabayashi, T. et al. (1998) JID. 177:1622.

Long Name

Acylation Stimulating Protein

Alternate Names

C3a des Arg

UniProt

P01024

Additional ASP/C3a desArg Products

Product Documents for Recombinant Human ASP/C3a desArg Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 9620-C3 (with carrier)
Download SDS 9620-C3/CF (carrier free)

Product Specific Notices for Recombinant Human ASP/C3a desArg Protein

For research use only

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