Recombinant Human Attractin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7238-AT

R&D Systems, part of Bio-Techne
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7238-AT-050
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Key Product Details

Source

CHO

Accession #

NP_647538

Conjugate

Unconjugated

Applications

Bioactivity

View all Attractin Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Attractin protein
Human Attractin
(Ala84-Gln1272)
Accession # NP_647538

HPGGGSGGGSGGGS

 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala84

Predicted Molecular Mass

135 kDa

SDS-PAGE

150-180 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When recombinant mouse Agouti is coated at 1 μg/mL, Recombinant Human Attractin binds with apparent KD <40 nM.

Formulation, Preparation and Storage

7238-AT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Attractin

Attractin, also known as DPPT-L and Mahogany, is a transmembrane glycoprotein with functions in hair pigmentation, axon myelination, energy balance regulation, and immune activation (1, 2). Mature human Attractin consists of a 1196 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 129 aa cytoplasmic domain (3). Within the ECD, it shares 95% aa sequence identity with mouse and rat Attractin. Alternative splicing generates an approximately 175 kDa secreted soluble isoform that corresponds to the ECD of the transmembrane form (4, 5). Attractin is expressed in activated T cells, monocytes, hair follicle melanocytes, brain (notably the hypothalamus, hippocampus, and substantia nigra), and adipose tissue (3, 6-9). Activated T cells release soluble Attractin which then co-stimulates T cell proliferation (3, 4). Attractin also functions with Melanocortin 1R as a co-receptor for Agouti and, as a soluble molecule, can neutralize the bioactivity of Agouti on hair follicle melanocytes (7, 8, 10, 11). Similarly, mutations in Attractin gene suppresses the diet-induced obesity which is characteristic of Agouti overexpressing mice (1, 7). In the CNS, Attractin plays a role in axon myelination, neurite branching on differentiating neurons, and protection from neurotoxins (1, 9, 12). It is elevated in the CSF in high grade malignant astrocytoma and promotes glioma cell migration (14).

References

  1. Gunn, T.M. et al. (2001) Genetics 158:1683.
  2. Friedrich, D. et al. (2007) Biol. Chem. 388:155.
  3. Duke-Cohan, J.S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:11336.
  4. Duke-Cohan, J.S. et al. (1996) J. Immunol. 156:1714.
  5. Tang, W. et al. (2000) Proc. Natl. Acad. Sci. USA 97:6025.
  6. Wrenger, S. et al. (2006) J. Leukoc. Biol. 80:621.
  7. Nagle, D.L. et al. (1999) Nature 398:148.
  8. He, L. et al. (2001) Nat. Genet. 27:40.
  9. Paz, J. et al. (2007) Neurobiol. Aging 28:1446.
  10. Hida, T. et al. (2009) Pigment Cell Melanoma Res. 22:623.
  11. Gunn, T.M. et al. (1999) Nature 398:152.
  12. Kuramoto, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:559.
  13. Tang, W. and J.S. Duke-Cohan (2002) J. Neuropathol. Exp. Neurol. 61:767.
  14. Khwaja, F.W. et al. (2006) Clin. Cancer Res. 12:6331.

Alternate Names

ATRN, DPPT-L, Mahogany Protein, MGCA

Entrez Gene IDs

8455 (Human); 11990 (Mouse); 83526 (Rat)

Gene Symbol

ATRN

UniProt

NP_647538

Additional Attractin Products

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Product Specific Notices for Recombinant Human Attractin Protein, CF

For research use only

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