Recombinant Human B3GNT4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7696-GT

R&D Systems, part of Bio-Techne
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7696-GT-020
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Key Product Details

Source

CHO

Accession #

Q9C0J1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Beta-1,3-N-Acetylglucosaminyltransferase 4/B3GNT4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Beta-1,3-N-Acetylglucosaminyltransferase 4/B3GNT4 protein
Ala56-Arg378, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala56

Predicted Molecular Mass

37 kDa

SDS-PAGE

40-55 kDa, reducing conditions

Activity

Measured by its ability to transfer N-acetylglucosamine from UDP-GlcNAc to beta-lactose.
The specific activity is >10 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7696-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Beta-1,3-N-Acetylglucosaminyltransferase 4/B3GNT4

beta1,3-Linked GlcNAc residues are present in the backbone of various biologically important glycans which are involved in many essential biological functions such as keratan sulfate synthesis in corneal tissue (1). The addition of such residues are catalyzed by a family of beta1,3-N-acetylglucosaminyltransferases, that includes at least eight members (15). All of them are type II Golgi resident transmembrane proteins and have high homology to the beta1,3-galactosyltransferase family. beta1,3-N-acetylglucosaminyltransferase 4 or beta3GNT4 is involved in polylactosamine synthesis and is mainly expressed in brain tissues such as whole brain, hippocampus, amygdala, cerebellum and caudate nucleus (6). The enzymatic activity of the recombinant protein was determined using a phosphatase coupled assay (7).

References

  1. Seko, A. and Yamashita, K. (2005) Glycobiology 15:943.
  2. Kataoka, K. and Huh, N.H. (2002) Biochem. Biophys. Res. Commun. 294:843.
  3. Iwai,T. et al. (2002) J. Biol. Chem. 277:12802.
  4. Togayachi, A. et al. (2001) J. Biol. Chem. 276:22032.
  5. Sasaki, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14294.
  6. Shiraishi, N. et al. (2001) J. Biol. Chem. 276:3498.
  7. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4

Alternate Names

BGnT-4

Entrez Gene IDs

79369 (Human); 231727 (Mouse); 288752 (Rat)

Gene Symbol

B3GNT4

UniProt

Q9C0J1 (Human)

Additional Beta-1,3-N-Acetylglucosaminyltransferase 4/B3GNT4 Products

Product Documents for Recombinant Human B3GNT4 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human B3GNT4 Protein, CF

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