Mouse myeloma cell line, NS0-derived human beta-1,4-Galactosyltransferase 1/B4GalT1 protein
Gly44-Ser398, with an N-terminal 6-His tag and a Y285L mutation

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

40 kDa

SDS-PAGE

45-60 kDa, reducing conditions

Activity

Measured by its ability to transfer N-Acetyl-D-galactosamine from UDP-GalNAc to N-Acetyl-D-glucosamine.
The specific activity is >2,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7040-GT

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Background: beta-1,4-Galactosyltransferase 1/B4GalT1

beta‑1,4‑Galactosyltransferase 1, also known as B4GalT1, is one of seven beta‑1,4 galactosyltransferases that transfer galactose to acceptor sugars including GlcNAc, Glc and Xyl (1, 2). A single point mutation, Y285L, in beta4GalT1 causes a functional switch in activity from Gal‑transferase activity to GalNAc‑transferase activity (3).The recombinant human B4GalT1(Y285L) can be used to synthesize lacdiNAc-terminated glycoconjugates, terminal glycan modifications found on many glycoproteins, including activation receptors of natural killer cells, NKR-P1 and CD69 (4). The enzymatic activity of the recombinant human B4GalT1 (Y285L) was assayed using a phosphatase-coupled method (5).

References

Guo, S. et al. (2001) Glycobiology 11:813.
Yamaguchi, N. and Fukuda, M.N. (1995) J. Biol. Chem. 270:12170.
Ramakrishnan, B. and Qasba, P.K. (2002) J. Biol. Chem. 277:20833.
Do, K.Y. et al. (1997) Glycobiology 7:183.
Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, Polypeptide 1

Alternate Names

GGTB2

Entrez Gene IDs

2683 (Human); 14595 (Mouse); 24390 (Rat)

Gene Symbol

B4GALT1

UniProt

P15291

Additional beta-1,4-Galactosyltransferase 1/B4GalT1 Products

Product Documents for Recombinant Human B4GalT1 (Y285L) Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Recombinant Human B4GalT1 (Y285L) Protein, CF

For research use only

Citations
Reviews

Recombinant Human B4GalT1 (Y285L) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7040-GT

Key Product Details

Source

Accession #

Conjugate

Applications

View all beta-1,4-Galactosyltransferase 1/B4GalT1 Proteins and Enzymes »

Product Specifications