Recombinant Human B4GalT2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7530-GT

R&D Systems, part of Bio-Techne
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7530-GT-020
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Key Product Details

Source

NS0

Accession #

O60909

Conjugate

Unconjugated

Applications

Enzyme Activity

View all beta-1,4-Galactosyltransferase 2/B4GalT2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human beta-1,4-Galactosyltransferase 2/B4GalT2 protein
Ser43-Gly372, with C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser43

Predicted Molecular Mass

38 kDa

SDS-PAGE

44-55 kDa, reducing conditions

Activity

Measured by its ability to transfer galactose from UDP-galactose to glucose.
The specific activity is >80 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7530-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: beta-1,4-Galactosyltransferase 2/B4GalT2

There are seven beta-1,4-galactosyltransferases that transfer galactose in a beta-1,4 linkage to acceptor sugars including GlcNAc, Glc, and Xyl. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7 (1). All of these enzymes are expressed as type II membrane proteins in Golgi apparatus except beta4GalT1, which can be expressed as a secreted form in lactating mammary tissues due to an alternative transcription initiation site (2, 3). beta4GalT2 is responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids (4). It can also produce lactose. Its substrate specificity is affected by alpha-lactalbumin, but it is not expressed in lactating mammary tissue (5). Recently, beta4GalT2 has been shown to form a complex with GlcAT-P to synthesize HNK-1 carbohydrate in the nervous system (6). The activity of this enzyme has been measured with a phosphatase-coupled method (7).

References

  1. Amado, M. et al. (1999) Biochim. Biophys. Acta. 1473:35.
  2. Appert, H.E. et al. (1986) Biochem. Biophys. Res. Commun.138:224.
  3. Mengle-Gaw,L. et al. (1991) Biochem. Biophys. Res.Commun. 176:1269.
  4. Guo, S. et al. (2001) Glycobiology 11:813.
  5. Almeida, R. et al. (1997) J. Biol. Chem. 272:31979.
  6. Kouno, T. et al. (2011) J. Biol. Chem. 286:31337.
  7. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, Polypeptide 2

Alternate Names

beta-1,4-Galactosyltransferase 2, Beta-1,4-GalTase 2

Entrez Gene IDs

8704 (Human); 53418 (Mouse); 313536 (Rat)

Gene Symbol

B4GALT2

UniProt

O60909 (Human)

Additional beta-1,4-Galactosyltransferase 2/B4GalT2 Products

Product Documents for Recombinant Human B4GalT2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human B4GalT2 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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