Recombinant Human B7-H6 Fc Chimera Biotinylated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT7144

R&D Systems, part of Bio-Techne
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BT7144-050
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Key Product Details

Source

NS0

Accession #

NP_001189368

Structure / Form

Disulfide-linked homodimer

Conjugate

Biotin

Applications

Bioactivity

View all B7-H6 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human B7-H6 protein
Human B7-H6
(Met1-Ser262)
Accession # NP_001189368		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp25

Predicted Molecular Mass

53 kDa (unlabeled)

SDS-PAGE

74-85 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NKp30 Fc Chimera (Catalog # 1849-NK) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human B7‑H6 Fc Chimera that produces 50% of the optimal binding response is 15-75 ng/mL.

Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
The ED50 for this effect is 0.6-3 μg/mL

Scientific Data Images for Recombinant Human B7-H6 Fc Chimera Biotinylated Protein, CF

Recombinant Human B7-H6 Fc Chimera Biotinylated Protein Binding Activity

Recombinant Human B7-H6 Fc Chimera Biotinylated Protein Binding Activity

When Recombinant Human NKp30 Fc Chimera (Catalog # 1849-NK) is coated at 1 µg/mL, Biotinylated Recombinant Human B7-H6 Fc Chimera (Catalog # BT7144) binds with an ED50 of 15-75 ng/mL.

Formulation, Preparation and Storage

BT7144
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H6

B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).

References

  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
  4. Li, Y. et al. (2011) J. Exp. Med. 208:703.
  5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
  6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
  7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.

Long Name

B7 Homolog 6

Alternate Names

B7H6, DKFZp686I21167, DKFZp686O24166, NCR3LG1

Entrez Gene IDs

374383 (Human); 102121659 (Cynomolgus Monkey)

Gene Symbol

NCR3LG1

UniProt

NP_001189368

Additional B7-H6 Products

Product Documents for Recombinant Human B7-H6 Fc Chimera Biotinylated Protein, CF

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Product Specific Notices for Recombinant Human B7-H6 Fc Chimera Biotinylated Protein, CF

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