Recombinant Human BACE-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4097-ASB

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
4097-ASB-020
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q9Y5Z0-1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all BACE-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human BACE-2 protein
Phe29-Pro466, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe29 & Ala63

Predicted Molecular Mass

49 kDa

SDS-PAGE

48-60 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010).
The specific activity is >70 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human BACE-2 Protein, CF

Recombinant Human BACE-2 Protein Enzyme Activity

Recombinant Human BACE-2 Protein Enzyme Activity

Recombinant Human BACE-2 (Catalog # 4097-ASB) is measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010).
Recombinant Human BACE-2 Protein SDS-PAGE

Recombinant Human BACE-2 Protein SDS-PAGE

2 μg/lane of Recombinant Human BACE‑2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at approximately 55 kDa under R conditions.

Formulation, Preparation and Storage

4097-ASB
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: BACE-2

BACE-2 (Beta secretase 2) is an aspartic protease that shares 48% sequence identity with BACE-1 in the extracellular catalytic domains. BACE-1 is the putative beta secretase for the generation of the A beta peptide in neurons (1). BACE-2 differs from BACE-1 in several aspects, including proenzyme activation, substrate preference, transcriptional regulation, and expression pattern (2, 3). Unlike BACE-1, BACE-2 activity does not contribute to Alzheimer's disease pathogenesis (4) but has been shown to play a key role in insulin receptor trafficking in the pancreas where it is expressed in beta-cells (5,6). BACE-2 affects glucose tolerance and was suggested as a promising target for improving beta-cell function in diabetes (7). Recombinant human BACE-2 was expressed without its C terminal transmembrane and cytosolic domains, resulting in its secretion from NS0 cells.

References

  1. Cai, H. et al. (2001) Nature Neurosci. 4:233.
  2. Hussain, I. et al. (2001) J. Biol. Chem. 276:23322.
  3. Ostermann, N. et al. (2006) J. Mol. Biol. 355:249.
  4. Sun, X. et al. (2006) FASEB J. 19:739.
  5. Esterhazy. D. et al. (2011) Cell Metab. 14:365.
  6. Casas, S. et al. (2010). Am. J. Physiol. Endocrinol. Metab. 299:E1087.
  7. Alcarraz-Vizan, G. et al. (2017). Cell Mol. Life Sci. 74:2827.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 2

Alternate Names

BACE2

Entrez Gene IDs

25825 (Human); 56175 (Mouse); 288227 (Rat)

Gene Symbol

BACE2

UniProt

Q9Y5Z0-1

Additional BACE-2 Products

Product Documents for Recombinant Human BACE-2 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human BACE-2 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.