Recombinant Human BAI2 Protein Fc Chimera, CF

R&D Systems, part of Bio-Techne | Catalog # 9338-BA

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

O60241-3

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BAI2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BAI2 protein
Human BAI2
(Phe21-Asp911 )
Accession # O60241-3		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe21

Predicted Molecular Mass

123 kDa

SDS-PAGE

128-200 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human C1qL3 (Catalog # 9115-TN) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera that produces 50% of the optimal binding response is approximately 25-150 ng/mL.

Scientific Data Images for Recombinant Human BAI2 Protein Fc Chimera, CF

Recombinant Human BAI2 Protein Fc Chimera Bioactivity

Recombinant Human BAI2 Protein Fc Chimera Bioactivity

When Recombinant Human C1qL3 (Catalog # 9115-TN) is coated at 2 µg/mL (100 µL/well), Recombinant Human BAI2 Fc Chimera (Catalog # 9338-BA) binds with an ED50 of 25-150 ng/mL.

Formulation, Preparation and Storage

9338-BA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAI2

Brain angiogenesis inhibitor 2 (BAI2), along with BAI1 and BAI3, form a subfamily of brain specific adhesion GPCRs. They are large, glycosylated molecules, approximately 180-220 kDa, and play a role in neuronal synapse formation and maintenance. Mature human BAI2 consists of a 903 amino acid (aa) N-terminal extracellular domain (ECD) containing four TSP1 domains, a hormone binding domain (HBD), a GAIN domain and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 410 aa C-terminal cytoplasmic domain (1). Within the N-terminal ECD, human BAI2 shares 96% aa sequence identity with both mouse and rat BAI2. BAI2 is expressed primarily in neurons and astrocytes of the hippocampus and cerebral cortex in the brain (2, 3). To become functional, BAI2 might require activation by cleavage at the GPS domain (4). BAI family members are known to bind the C1q-like complement (C1qL) family via thrombospondin type 1 repeats (TSRs) which are conserved between BAI members (5). BAI2 has been shown to suppress expression of VEGF leading to increased neurogenesis in the dentate gyrus of the hippocampus (6).

References

  1. Duman, J.G. et al. (2016) Neural Plast. 2016:8301737.
  2. Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
  3. Kee, H.J. et al. (2002) J. Cereb. Blood Flow Metab. 22:1054.
  4. Daisuke, O. et al. (2010) J. Recept Signal Transduct Res 30:143.
  5. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
  6. Jeong, B.C. et al. (2016) FEBS Lett 580:669.

Long Name

Brain-specific Angiogenesis Inhibitor 2

Alternate Names

brain-specific angiogenesis inhibitor 2, Brain-specific angiongenesis inhibitor-2

Entrez Gene IDs

576 (Human); 230775 (Mouse); 313058 (Rat)

Gene Symbol

ADGRB2

UniProt

O60241-3

Additional BAI2 Products

Product Documents for Recombinant Human BAI2 Protein Fc Chimera, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human BAI2 Protein Fc Chimera, CF

For research use only

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