Recombinant Human C1qL3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9115-TN

R&D Systems, part of Bio-Techne
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9115-TN-050
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Key Product Details

Source

E. coli

Accession #

Q5VWW1

Structure / Form

Noncovalently-linked homotrimer.

Conjugate

Unconjugated

Applications

Bioactivity

View all C1qL3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human C1qL3 protein
Ser122 - Asp255, with an N-terminal Met and 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

14 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human C1qL3 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera that produces 50% of the optimal binding response is approximately 25-150 ng/mL.

Scientific Data Images for Recombinant Human C1qL3 Protein, CF

Recombinant Human C1qL3 Protein Bioactivity

Recombinant Human C1qL3 Protein Bioactivity

When Recombinant Human C1qL3 (Catalog # 9115-TN) is coated at 2 µg/mL, Recombinant Human BAI2 Fc Chimera binds with a typical ED50 of 25-150 ng/mL.

Formulation, Preparation and Storage

9115-TN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Reconstitution
Reconstitute at 500 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1qL3

C1qTNF proteins constitute a highly conserved family of Acrp30/Adiponectin paralogs that share a modular organization comprising an N-terminal signal peptide, a short variable region, a collagenous domain and a C-terminal globular domain. C1qTNF proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. C1qTNF3, also known as CORS26, is predominantly expressed in cartilage and is induced in mature adipocytes. C1qTNF10, also known as C1qL2, is a protein that shows 28% - 30% sequence identity with C1q subunits A, B, and C. It is predicted to contain one collagen-like and C1q domain. Mouse and human C1qTNF10 share over 90% amino acid sequence identity.

Long Name

Complement Component 1, Q Subcomponent-Like 3

Alternate Names

C1q-like 3, C1ql, C1qTNF13, CTRP13, K100

Entrez Gene IDs

389941 (Human); 227580 (Mouse); 680404 (Rat)

Gene Symbol

C1QL3

UniProt

Q5VWW1

Additional C1qL3 Products

Product Documents for Recombinant Human C1qL3 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human C1qL3 Protein, CF

For research use only

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