Recombinant Human BCAT2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9537-BA

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9537-BA-020
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

O15382-1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all BCAT2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human BCAT2 protein
Ala28-Val392
with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

42 kDa

SDS-PAGE

37-42 kDa, reducing conditions

Activity

Measured by its ability to convert leucine and alpha-ketoglutarate to alpha-ketoisocaproate and glutamate.
The specific activity is >3,500 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human BCAT2 Protein, CF

Recombinant Human BCAT2 Protein Enzyme Activity

Recombinant Human BCAT2 Protein Enzyme Activity

Recombinant Human BCAT2 (Catalog # 9537-BA) is measured by its ability to convert leucine and alpha-ketoglutarate to alpha-ketoisocaproate and glutamate.

Formulation, Preparation and Storage

9537-BA
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: BCAT2

Branched-chain-amino-acid aminotransferases (BCATs) are enzymes that catalyze the first reaction in the catabolism of the essential branched-chain amino acids leucine, isoleucine, and valine to their respective keto-acids while concurrently producing glutamate. BCATs belong to the class-IV pyridoxal-phosphate-dependent (PLP-dependent) aminotransferase family of enzymes (1). There are two BCAT isozymes in humans and mammals, a mitochondrial form known as BCATm or BCAT2 and a cytosolic form known as BCATc or BCAT1 that share 55% sequence identity. In humans and rodents, BCATc is almost exclusively present in the nervous system (2,3) while BCATm is constitutively expressed in most tissues and is generally thought to be important in body nitrogen metabolism (1,2). The 41 kDa human BCATm exists as an active homodimer and has a unique CXXC active site near the dimerization domain (4). Knockouts display decreased adiposity and obesity through alterations of leucine-dependent mTOR signaling making it a potential therapeutic target for obesity (5). BCATm can be regulated by oxidative stress, interfering with its ability to interact with protein disulfide isomerase (6). BCATm overexpression in the brain is detectable in Alzheimers disease and dementia (7,8) suggesting it plays an important role in glutamate toxicity, a key pathogenic feature of these diseases.

References

  1. Hutson, S. (2001) Prog. Nucleic. Acid Res. Mol. Biol. 70:175.
  2. Suryawan, A. et al. (1998) Am. J. Clin. Nutr. 68:72.
  3. Hall, T.R. et al. (1993) J. Biol. Chem. 268:3092.
  4. Yennawar, N. H. (2006) J. Biol. Chem. 281:39660. (BCATm)
  5. She, P. et al. (2007) Cell Metab. 6:181.
  6. El Hindy, M. et al. (2014) Antioxid. Redox Signal 20:2497.
  7. Ashby, E.L. et al. (2017) Neurochem. Res. 42:306.
  8. Hull, J. et al. (2015) J. Alzheimers Dis. 45:891.

Long Name

Branched Chain Amino Acid Transaminase 2

Alternate Names

BCAM, BCATM, BCT2, Eca40

Entrez Gene IDs

587 (Human); 12036 (Mouse); 64203 (Rat)

Gene Symbol

BCAT2

UniProt

O15382-1

Additional BCAT2 Products

Product Documents for Recombinant Human BCAT2 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human BCAT2 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.