Recombinant Human Bcl-2 (minus C-Terminus) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 827-BC
Key Product Details
Product Specifications
Source
E. coli-derived human Bcl-2 protein
Ala2-Asp211, with a C-terminal 10-His tag
Ala2-Asp211, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala2
Predicted Molecular Mass
24.5 kDa
SDS-PAGE
27 kDa, under reducing and non-reducing conditions
Activity
Measured by its ability to inhibit Caspase-8-cleaved BID-mediated release of cytochrome c from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog # MCTC0) to quantify cytochrome c.
The typical EC50 is less than 500 nM in the presence of 54 nM Recombinant Human BID Caspase-8-cleaved (Catalog # 882-B8).
Optimal dilutions should be determined by each laboratory for each application.
The typical EC50 is less than 500 nM in the presence of 54 nM Recombinant Human BID Caspase-8-cleaved (Catalog # 882-B8).
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
827-BC
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, KCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Bcl-2
Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
References
- Gross, A. et al. (1999) Genes and Develop. 13:1899.
- Kroemer, G. (1997) Nature Med. 3:614.
Long Name
B Cell Lymphoma/Leukemia 2
Alternate Names
Bcl2
Gene Symbol
BCL2
UniProt
Additional Bcl-2 Products
Product Documents for Recombinant Human Bcl-2 (minus C-Terminus) Protein, CF
Product Specific Notices for Recombinant Human Bcl-2 (minus C-Terminus) Protein, CF
For research use only
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