Recombinant Human BIM (Long Form) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1325-BL

R&D Systems, part of Bio-Techne
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1325-BL-050
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Key Product Details

Source

E. coli

Accession #

NP_006529

Conjugate

Unconjugated

Applications

Bioactivity

View all BIM Long (BIM L) Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human BIM Long (BIM L) protein
Ala2-Arg120, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

14.3 kDa

SDS-PAGE

13-16 kDa and 30-35 kDa, reducing conditions

Activity

Measured by its ability to neutralize anti-apoptotic Bcl-2 family member inhibition of Recombinant Human BID Caspase-8-cleaved (Catalog # 882-B8)-mediated Cytochrome c release from isolated mitochondria. Release of Cytochrome c is quantified using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog # MCTC0).
The typical EC50 value in this assay is 0.1-2 µM.
Optimal dose should be determined for each application.

Formulation, Preparation and Storage

1325-BL
Formulation Supplied as a 0.2 μm filtered solution in HEPES-KOH, DTT, KCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: BIM Long (BIM L)

BIML (also known as Bod) is one of several splice variants of BIM, a pro-apoptotic protein belonging to the BH3 domain-only subgroup of Bcl-2 family members. BIML is thought to promote apoptosis by binding and inhibiting the activity of anti-apoptoticBcl-2 family members, thereby inducing the release of cytochrome c from mitochondria. BIML is normally sequestered in an inactive conformation from anti-apoptotic Bcl-2 family members through binding to the microtubule-associated dynein motor complex.Certain apoptotic stimuli release BIML from microtubules to neutralize anti-apoptotic Bcl-2 family members, allowing for the initiation of apoptosis.

References

  1. O'Connor, L. et al. (1998) EMBO J. 17:384.
  2. Puthalakath, H. et al. (1999) Mol. Cell. 3:287.
  3. Miyashita, T. (2001) FEBS Letters 509:135.
  4. Strasser, A. (2000) Ann N Y Acad Sci. 917:541.
  5. Marani, M. et al. (2002) Mol. Cell. Biol. 22:3577.

Long Name

Bcl-2 Interacting Mediator of Cell Death

Alternate Names

BCL2L11

Entrez Gene IDs

10018 (Human); 12125 (Mouse)

Gene Symbol

BCL2L11

UniProt

NP_006529

Additional BIM Long (BIM L) Products

Product Documents for Recombinant Human BIM (Long Form) Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human BIM (Long Form) Protein, CF

For research use only

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