Recombinant Human BTN2A1/Butyrophilin 2A1 Fc Avi Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10909

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q7KYR7.3

Structure / Form

Disulfide-linked homodimer. Biotinylated via Avi-tag.

Conjugate

Biotin

Applications

Bioactivity

View all BTN2A1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human BTN2A1 protein
Human BTN2A1
(Gln29-Ala248)
Accession # Q7KYR7.3		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Protein identify confirmed by mass spectromety.

Predicted Molecular Mass

53 kDa

SDS-PAGE

65-80 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD277/BTN3A1 Fc Chimera Protein (Catalog # 8539-BT) is immobilized at 10.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human BTN2A1 Fc Chimera Avi-tag (Catalog #AVI10909) that produces 50% of the binding response is 1.00-6.00 μg/mL.

Scientific Data Images for Recombinant Human BTN2A1/Butyrophilin 2A1 Fc Avi Protein, CF

Biotinylated Recombinant Human BTN2A1 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human BTN2A1 Fc Chimera Avi-tag Protein (Catalog # AVI10909) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa to 130-160 kDa, respectively.

Formulation, Preparation and Storage

AVI10909
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: BTN2A1

Butyrophilin 2A1 (BTN2A1) is a type I transmembrane glycoprotein of the butyrophilin family within the Ig-superfamily. There are over 13 Butyrophilin and the closely related butyrophilin-like (BTNL) molecules and they have been identified as immune checkpoint receptors involved in modulating T cell function (1,2). Mature human BTN2A1 consists of an extracellular domain (ECD) with two immunoglobulin-like domains (one IgV and one IgC), a transmembrane segment, and a cytoplasmic region with a B30.2 domain (3). Alternative splicing generates additional isoforms of BTN2A1 that lack the first Ig-like domain or the transmembrane segment as well as isoforms with substitutions and deletions in the cytoplasmic region. BTN2A1 is widely expressed including on colonic epithelial cells, on immune cells, and in milk fat globules (4, 5). BTN2A1 binds to the C-type lectin DC-SIGN on monocyte-derived dendritic cells, and this interaction can be blocked by soluble gp130 from HIV (4). A polymorphism of BTN2A1 has been associated with metabolic syndromes, type II diabetes mellitus, chronic kidney disease, and hypertension (6, 7). Our Avi-tag Biotinylated BTN2A1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
  2. Afrache, H. et al. (2012) Immunogenetics 64:781.
  3. Tazi-Ahnini, R. et al. (1997) Immunogenetics 47:55.
  4. Malcherek, G. et al. (2007) J. Immunol. 179:3804.
  5. Cavaletto, M. et al. (2002) Proteomics 2:850.
  6. Oguri, M. et al.(2011) J. Med. Genet. 48:787.
  7. Horibe, H. et al. (2014) Mol. Med. Rep. 9:808.

Long Name

Butyrophilin Subfamily 2 Member A1

Alternate Names

BT2.1, BTF1

Entrez Gene IDs

11120 (Human)

Gene Symbol

BTN2A1

UniProt

Q7KYR7.3

Additional BTN2A1 Products

Product Documents for Recombinant Human BTN2A1/Butyrophilin 2A1 Fc Avi Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human BTN2A1/Butyrophilin 2A1 Fc Avi Protein, CF

For research use only

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