Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9058-BT

R&D Systems, part of Bio-Techne
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9058-BT-050
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Key Product Details

Source

HEK293

Accession #

Q7KYR7

Conjugate

Unconjugated

Applications

Bioactivity

View all BTN2A1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BTN2A1 protein
Gln29-Ala248, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln29 predicted

Predicted Molecular Mass

25 kDa

SDS-PAGE

39-44 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human BTN2A1 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human DC‑SIGN/CD209 Fc Chimera (Catalog # 161-DC) that produces 50% of the optimal binding response is approximately
0.1-0.5 μg/mL.

Scientific Data Images for Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF

Recombinant Human BTN2A1/Butyrophilin 2A1 Protein Bioactivity

Recombinant Human BTN2A1/Butyrophilin 2A1 Protein Bioactivity

When Recombinant Human BTN2A1/Butyrophilin 2A1 (Catalog # 9058-BT) is coated at 1 µg/mL, Recombinant Human DC-SIGN/CD209 Fc Chimera (Catalog # 161-DC) binds with a ED50 of 0.1-0.5 μg/mL.

Formulation, Preparation and Storage

9058-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN2A1

Butyrophilin 2A1 (BTN2A1) is an approximately 70 kDa widely expressed and variably glycosylated type I transmembrane glycoprotein (1). Mature human Butyrophilin 2A1 consisits of a 220 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 258 aa cytoplasmic domain (2). Alternative splicing generates additional isoforms of human Butyrophilin 2A1 that lack the first Ig-like domain or transmembrane segment as well as isoforms with substitutions and deletions in the cytoplasmic region. BTN2A1 is widely expressed including on colonic epithelial cells, on immune cells, and in milk fat globules (3, 4). It binds to the C-type lectin DC-SIGN on monocyte-derived dendritic cells, and this interaction can be blocked by soluble gp130 from HIV (3). The polymorphism of BTN2A1 has been associated with metabolic syndrome, type II diabetes mellitus, chronic kidney disease, and hypertension (5, 6).

References

  1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
  2. Tazi-Ahnini, R. et al. (1997) Immunogenetics 47:55.
  3. Malcherek, G. et al. (2007) J. Immunol. 179:3804.
  4. Cavaletto, M. et al. (2002) Proteomics 2:850.
  5. Oguri, M. et al.(2011) J. Med. Genet. 48:787.
  6. Horibe, H. et al. (2014) Mol. Med. Rep. 9:808.

Long Name

Butyrophilin Subfamily 2 Member A1

Alternate Names

BT2.1, BTF1

Entrez Gene IDs

11120 (Human)

Gene Symbol

BTN2A1

UniProt

Q7KYR7

Additional BTN2A1 Products

Product Documents for Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF

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