Recombinant Human BTN3A2 His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9514

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P78410-1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all BTN3A2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BTN3A2 protein
Human BTN3A2
(Gln30-Trp248)
Accession # P78410-1		 HHHHHH  Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

26 kDa

SDS-PAGE

27-37 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Sheep Anti-Human BTN3A1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7136) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human BTN3A2 His-tag Avi-tag (Catalog # AVI9514) that produces 50% of the optimal binding response is approximately 15-75 ng/mL.

Measured by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3+ T cells.
The ED50 for this effect is 1.5-9.0 μg/mL.

Scientific Data Images for Recombinant Human BTN3A2 His-tag Avi-tag Protein, CF

Recombinant Human BTN3A2 His-tag Avi-tag Protein SDS-PAGE

Recombinant Human BTN3A2 His-tag Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human BTN3A2 His-tag Avi-tag (Catalog # AVI9514) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 27-37 kDa.
Recombinant Human BTN3A2 His-tag Avi-tag Protein Binding Activity

Recombinant Human BTN3A2 His-tag Avi-tag Protein Binding Activity

When Sheep Anti-Human BTN3A1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7136) is immobilized at 1.0 µg/mL, 100 µL/well, the concentration of Biotinylated Recombinant Human BTN3A2 His-tag Avi-tag (Catalog # AVI9514) that produces 50% of the optimal binding response is approximately 15-75 ng/mL.

Formulation, Preparation and Storage

AVI9514
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN3A2

BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. It is postulated to be expressed on immune-related cells, as it has a structural similarity to MHC and CD80/CD86 molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region with one V-type Ig-like domain and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. A rodent counterpart to BTN3A2 has not been reported. BTN3A2 mRNA over-expression was associated with a good prognosis in relation to disease-free and overall survival in a cohort of 55 epithelial ovarian cancer (EOC) patients (1). Another study in a larger cohort of 199 high-grade EOC patients further confirmed that the protein expression of BTN3A2 in ovarian cancer tissues is positively correlated with the intraepithelial infiltration of CD4+ and CD8+ T cells (2), suggesting that BTN3A2 was a co-stimulatory molecule to modulate the infiltration of immune cells and thus the anti-cancer immunity. In consistent with previous publications, our in-house studies on BTN3A2 showed that BTN3A2 co-stimulated anti-CD3 induced IFN-gamma secretion on CD3+ cells.

References

  1. LePage C., et al. Cancer Epidemiol Biomarkers Prev (2008) 17:913.
  2. LePage C., et al. PLoSOne (2012) 7:e38541.

Long Name

Butyrophilin, Subfamily 3, Member A2

Alternate Names

BT3.2, BT3.3, BTF4

Entrez Gene IDs

11118 (Human); 294268 (Rat)

Gene Symbol

BTN3A2

UniProt

P78410-1

Additional BTN3A2 Products

Product Documents for Recombinant Human BTN3A2 His-tag Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human BTN3A2 His-tag Avi-tag Protein, CF

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