Recombinant Human BTNL3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9658-BT

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

Q6UXE8-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BTNL3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human BTNL3 protein
Human BTNL3
(Gln18-Ser237)
Accession # Q6UXE8-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln18 inferred from enzymatic pyroglutamate treatment revealing Trp19

Predicted Molecular Mass

51 kDa

SDS-PAGE

53-63 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 1-6 μg/mL.

Scientific Data Images for Recombinant Human BTNL3 Fc Chimera Protein, CF

Recombinant Human BTNL3 Fc Chimera Protein Bioactivity

Recombinant Human BTNL3 Fc Chimera Protein Bioactivity

Recombinant Human BTNL3 Fc Chimera (Catalog # 9658-BT) inhibits IFN‑gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-6 μg/mL.

Formulation, Preparation and Storage

9658-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTNL3

Butyrophilin-like 3 (BTNL3) is a member of the BTN/MOG Ig-superfamily which is coded by the BTNL3 gene, also known as COLF4100 (1). BTNL3 is a 466 amino acid (aa) type I transmembrane protein that contains a 220 aa extracellular domain (ECD), a 21 aa helical transmembrane domain, and a 208 aa cytoplasmic domain. The ECD features both an IgV and IgC domain, which is commonplace in BTN/BTNL proteins, but the IgC domain is relatively weak (2-5). The ECD of human BTNL3 shares only 37% and 34% aa sequence identity with mouse (referred to as BTNL1) and rat, respectively. Human BTNL3 mRNA has been identified in many tissues including small intestine, colon, liver, lung, bone marrow and spleen tissue, and it is highly expressed in neutrophils (2-6). BTNL3 expression has also been found to be down-regulated in colon cancer tumors (7). The specific function of BTNL3, however, has yet to be fully elucidated. R&D in house data indicate that BTNL3 protein inhibits the secretion of IFN-gamma from anti-CD3 activated human T cells.

References

  1. Shibui, A. et al. (1999) J. Hum. Genet. 44:249.
  2. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:5596.
  3. Arnett, H.A. et al. (2009) Cytokine 46:370.
  4. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  5. Rhodes, D. et al. (2016) Annu. Rev. Immunol. 34:151.
  6. Guo, Y and Wang, AY (2015) Front. Immunol. 6:421.
  7. Lebrero-Fernandez, C. et al. (2016) Immun. Inflammation Dis. 4:191.

Long Name

Butyrophilin-like 3

Alternate Names

BTN9.1, BTNLR, COLF4100

Entrez Gene IDs

10917 (Human); 100038862 (Mouse); 407781 (Rat)

Gene Symbol

BTNL3

UniProt

Q6UXE8-1

Additional BTNL3 Products

Product Documents for Recombinant Human BTNL3 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human BTNL3 Fc Chimera Protein, CF

For research use only

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