Recombinant Human C1qR1/CD93 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2379-CD

R&D Systems, part of Bio-Techne
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2379-CD-050
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Key Product Details

Source

NS0

Accession #

Q9NPY3

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all C1qR1/CD93 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human C1q R1/CD93 protein
Human C1q R/CD93
(Ala24-Lys580)
Accession # Q9NPY3		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala24

Predicted Molecular Mass

84.7 kDa (monomer)

SDS-PAGE

110-130 kDa, reducing conditions

Activity

Measured by its ability to induce TNF-alpha secretion by THP-1 human acute monocytic leukemia cells.
The ED50 for this effect is 6-30 μg/mL.

Formulation, Preparation and Storage

2379-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1qR1/CD93

C1q  R1, also known as CD93 and C1q Rp, is an approximately 125 kDa transmembrane glycoprotein that is involved in various aspects of inflammatory reactions (1). Mature human CD93 consists of a 557 amino acid (aa) extracellular domain (ECD) with one C‑type lectin domain, four tandem EGF‑like domains, and a mucin‑like domain, followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (2, 3). Within the ECD, human CD93 shares 65% aa sequence identity with mouse and rat CD93. It is distinct from the 60 kDa Calreticulin which is likewise known as C1q  R1 (4, 5). Unlike Calreticulin, and despite its name, C1q  R1/CD93 does not appear to bind the complement protein C1q (3, 5). CD93 is expressed by vascular endothelial cells (5) and by a variety of hematopoietic cells (3‑9). Various sized fragments of soluble CD93 (50‑75 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a residual stub in the membrane (11‑13). Cross‑linking of cell surface CD93 enhances phagocytosis by monocytes and enhances the uptake of apoptotic cells in vivo (10, 15). Soluble CD93 promotes the differentiation of monocytes to macrophages, phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands (12, 14). It is required for plasma cell longevity in the bone marrow and for the resulting extended humoral response (9).

References

  1. Greenlee-Wacker, M.C. et al. (2012) Curr. Drug Targets 13:411.
  2. Nepomuceno, R.R. et al. (1997) Immunity 6:119.
  3. Steinberger, P. et al. (2002) J. Leukoc. Biol. 71:133.
  4. Nepomuceno, R.R. and A.J. Tenner (1998) J. Immunol. 160:1929.
  5. McGreal, E.P. et al. (2002) J. Immunol. 168:5222.
  6. Lovik, G. et al. (2001) Scand. J. Immunol. 53:410.
  7. Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441.
  8. Ikewaki, N. et al. (2010) J. Clin. Immunol. 30:723.
  9. Chevrier, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3895.
  10. Norsworthy, P.J. et al. (2004) J. Immunol. 172:3406.
  11. Bohlson, S.S. et al. (2005) J. Immunol. 175:1239.
  12. Greenlee, M.C. et al. (2009) Inflamm. Res. 58:909.
  13. Greenlee-Wacker, M.C. et al. (2011) J. Immunol. 187:3353.
  14. Jeon, J.W. et al. (2010) J. Immunol. 185:4921.
  15. Nepomuceno, R.R. et al. (1999) J. Immunol. 162:3583.

Long Name

Complement Component C1q Receptor

Alternate Names

AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Gene Symbol

CD93

UniProt

Q9NPY3

Additional C1qR1/CD93 Products

Product Documents for Recombinant Human C1qR1/CD93 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human C1qR1/CD93 Fc Chimera Protein, CF

For research use only

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