Recombinant Human C1qTNF1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9268-TN

R&D Systems, part of Bio-Techne
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9268-TN-050
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Key Product Details

Source

NS0

Accession #

Q9BXJ1

Conjugate

Unconjugated

Applications

Bioactivity

View all CTRP1/C1qTNF1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CTRP1/C1qTNF1 protein
Arg26-Pro281, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg26

Predicted Molecular Mass

30 kDa

SDS-PAGE

33-41 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human BAI3 is coated at 4 µg/mL, Recombinant Human C1qTNF1 (Catalog # 9268-TN) binds with an
ED50 = 0.6-3.6 µg/mL.

Scientific Data Images for Recombinant Human C1qTNF1 Protein, CF

Recombinant Human C1qTNF1 Protein Bioactivity

Recombinant Human C1qTNF1 Protein Bioactivity

When Recombinant Human BAI3 is coated at 4 µg/mL, Recombinant Human C1qTNF1 (Catalog # 9268-TN) binds with an ED50 of 0.6-3.6 µg/mL.

Formulation, Preparation and Storage

9268-TN
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP1/C1qTNF1

C1qTNF1 (CTRP1) is an approximately 35 kDa member of the C1q family of secreted proteins and plays a role in energy metabolism and inflammation (1, 2). C1qTNF1 contains a collagen-like region and one C1q-like domain (3). Mature human C1qTNF1 shares 80% aa sequence identity with mouse and rat C1qTNF1. Circulating levels of C1qTNF1 are elevated in obesity, hypertension, and diabetes but can be decreased in the serum of diet-induced obese mice (4-6). C1qTNF1 expression is up-regulated in atherosclerotic plaques or adipose tissue by oxidized LDL or inflammatory cytokines (3, 7, 8). In turn, it induces the expression of inflammatory cytokines (7, 9) and the up-reglation of adhesion proteins on vascular endothelial cells (8). Systemically administered C1qTNF1, in contrast, can limit tissue damage following myocardial infarction (9). In skeletal muscle, C1qTNF1 promotes fatty acid oxidation, energy expenditure, insulin sensitivity, and glucose uptake and glycolysis (6, 10). It also induces the proliferation of immature chondrocytes (11) and aldosterone synthesis in the adrenal cortex (4). R&D Systems in-house testing indicates that C1qTNF1 binds to BAI3, consistent with the reported interactions between BAI3 and C1qL proteins (12).

References

  1. Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
  2. Seldin, M.M. et al. (2014) Rev. Endocr. Metab. Disord. 15:111.
  3. Kim, K.-Y. et al. (2006) FEBS Lett. 580:3953.
  4. Jeon, J.H. et al. (2008) FASEB J. 22:1502.
  5. Xin, Y. et al. (2014) Endocr. J. 61:841.
  6. Peterson, J. M. et al. (2012) J. Biol. Chem. 287:1576.
  7. Wang, X.Q. et al. (2016) Atherosclerosis 250:38.
  8. Lu, L. et al. (2016) Eur. Heart J. 37:1762.
  9. Yuasa, D. et al. (2016) FASEB J. 30:1065.
  10. Han, S. et al. (2016) J. Nutr. Biochem. 27:43.
  11. Akiyama, H. et al. (2013) Mol. Cell. Endocrinol. 369:63.
  12. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.

Long Name

C1q And Tumor Necrosis Factor Related Protein 1

Alternate Names

C1qTNF1, ZSIG37

Entrez Gene IDs

114897 (Human); 56745 (Mouse); 303701 (Rat)

Gene Symbol

C1QTNF1

UniProt

Q9BXJ1

Additional CTRP1/C1qTNF1 Products

Product Documents for Recombinant Human C1qTNF1 Protein, CF

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Product Specific Notices for Recombinant Human C1qTNF1 Protein, CF

For research use only

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