Recombinant Human C1qTNF10/C1qL2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9189-TN

R&D Systems, part of Bio-Techne
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9189-TN-050
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Key Product Details

Source

E. coli

Accession #

Q7Z5L3

Structure / Form

Noncovalently-linked homotrimer

Conjugate

Unconjugated

Applications

Bioactivity

View all C1qTNF10/C1qL2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human C1qTNF10/C1qL2 protein
Phe154-Asp287, with an N-terminal Met and 6-His tag

Purity

>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

14 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human C1qTNF10/C1qL2 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Protein Fc Chimera (Catalog # 9338-BA) that produces 50% of the optimal binding response is approximately 0.075-0.45 μg/mL.

Scientific Data Images for Recombinant Human C1qTNF10/C1qL2 Protein, CF

Recombinant Human C1qTNF10/C1qL2 Protein Bioactivity

Recombinant Human C1qTNF10/C1qL2 Protein Bioactivity

When Recombinant Human C1qTNF10 (Catalog # 9189-TN) is coated at 2 μg/mL, 100 μL/well, Recombinant Human BAI2 Fc Chimera binds with a ED50 of 0.075-0.45 μg/mL.

Formulation, Preparation and Storage

9189-TN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP and Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1qTNF10/C1qL2

C1qTNF10 (CTRP10), also known as C1qL2, is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Mature human C1qTNF10 contains a collagen-like region and one C1q-like domain. It can form heterotrimers and higher order disulfide linked oligomers with C1qTNF1, C1qTNF11/C1qL4, and C1qTNF13/C1qL3 (3-6). Within the C1q-like domain, human C1qTNF10 shares 100% aa sequence identity with mouse and rat C1qTNF10. Similarly to C1qTNF11, C1qTNF13, and C1qTNF14/C1qL1, C1qTNF10 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (7). It is produced by mossy fibers in the hippocampus where it enhances the postsynaptic clustering of kainate-type glutamate receptors (KAR) on CA3 pyramidal neurons (8). This function is enhanced by the binding of C1qTNF10 to the KAR subunits GluK2 and GluK4 as well as to Neurexin-3 beta containing the exon 5b splice site insertion (8).

References

  1. Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
  2. Yuzaki, M. (2001) Eur. J. Neurosci. 32:191.
  3. Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
  4. Wei, Z. et al. (2011) J. Biol. Chem. 286:15652.
  5. Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
  6. Wong, G.W. et al. (2008) Biochem. J. 416:161.
  7. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
  8. Matsuda, K. et al. (2016) Neuron 90:752.

Long Name

C1q And Tumor Necrosis Factor Related Protein 10

Alternate Names

C1qL2

Entrez Gene IDs

165257 (Human); 226359 (Mouse)

Gene Symbol

C1QL2

UniProt

Q7Z5L3

Additional C1qTNF10/C1qL2 Products

Product Documents for Recombinant Human C1qTNF10/C1qL2 Protein, CF

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Product Specific Notices for Recombinant Human C1qTNF10/C1qL2 Protein, CF

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