Recombinant Human C1qTNF11/C1qL4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9758-TN

R&D Systems, part of Bio-Techne
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9758-TN-050
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Key Product Details

Source

E. coli

Accession #

Q86Z23

Conjugate

Unconjugated

Applications

Bioactivity

View all C1qL4 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human C1qL4 protein
Gly105-Asp238, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

13 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human C1qL4 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera (Catalog # 9338-BA) that produces 50% of the optimal binding response is 2-10 μg/mL.

Formulation, Preparation and Storage

9758-TN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1qL4

C1qTNF11 (CTRP11), also known as C1qL4, is an approximately 29 kDa member of the C1qTNF family of secreted proteins (1, 2). Mature human C1qTNF11/C1qL4 contains two distinct domains: a collagen-like region and one C1q-like domain, and can form disulfide-linked heteromers with C1qTNF14/C1qL1 (3, 4). The C1qTNF11/C1qL4 gene is conserved in human, chimpanzee, dog, mouse, rat, zebrafish, and frog. Within the C1q-like domain, human C1qTNF11/C1qL4 shares 100% amino acid (aa) sequence identity with that of mouse, monkey and rat. C1qTNF11/C1qL4 is expressed predominantly in testis and adipocytes, and also at a lower level in skeletal muscle and kidney (4), hippocampus, and cerebral cortex (5). Similar to C1qTNF13/C1qL3, C1qTNF10/C1qL2, and C1qTNF14/C1qL1, C1qTNF11/C1qL4 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (5-8). C1qTNF11/C1qL4 binding to BAI3 induces the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (5, 7). Our in house assay shows that C1qTNF11/C1qL4 also binds to BAI-2.  In addition, C1qTNF11/C1qL4 has been reported to promote angiogenesis via activation of the ERK pathway in HUVEC cells mediated by BAI3 (9). Conversely, in adipocytes, C1qTNF11/C1qL4 treatment decreased ERK1/2 signaling and inhibited adipogenesis (4).

References

  1. Ghebrehiwet, B. et al. (2012) Front. Immunol. 3: 52.
  2. Yuzaki, M. (2010) Eur. J. Neurosci. 32:191.
  3. Ressl, S. et al. (2015) Structure 23:688.
  4. Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
  5. Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
  6. Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
  7. Kakegawa, W. et al. (2015) Neuron 85:316.
  8. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
  9. Liu, F. et al. (2017) Mol. Cell. Biochem. 424:57.

Long Name

Complement Component 1, Q Subcomponent-Like 4

Alternate Names

C1qTNF11, CTRP11

Entrez Gene IDs

338761 (Human); 239659 (Mouse); 300220 (Rat)

Gene Symbol

C1QL4

UniProt

Q86Z23

Additional C1qL4 Products

Product Documents for Recombinant Human C1qTNF11/C1qL4 Protein, CF

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Product Specific Notices for Recombinant Human C1qTNF11/C1qL4 Protein, CF

For research use only

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