Recombinant Human Cadherin-6/KCAD Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2715-CA

R&D Systems, part of Bio-Techne
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2715-CA-050
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Key Product Details

Source

NS0

Accession #

P55285

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Cadherin-6/KCAD Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Cadherin-6/KCAD protein
Human Cadherin-6
(Thr22-Ala615 & Ser54-Ala615)
Accession # P55285		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr22 & Ser54

Predicted Molecular Mass

93.7 kDa (pro), 90 kDa (mature)

SDS-PAGE

120-140 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Caki-2 human clear cell carcinoma epithelial cells.
When 5 x 104 cells/well are added to Recombinant Human Cadherin-6 Fc Chimera coated plates (5 µg/mL with 100 µL/well), >30% of cells will adhere after 30 minutes at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.

Formulation, Preparation and Storage

2715-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-6/KCAD

Cadherin-6, also known as KCAD or K-Cadherin, is a 110-120 kDa type I transmembrane glycoprotein belonging to the classical Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Human Cadherin-6 is synthesized with an 18 amino acid (aa) signal peptide and a 35 aa N-terminal propeptide. The mature cell surface-expressed protein consists of a 562 amino acid (aa) extracellular domain (ECD) that contains five tandem Cadherin repeats, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (3). Within the ECD, human Cadherin-6 shares 96% and 98% aa sequence identity with mouse and rat Cadherin-10, respectively. Alternate splicing of the human Cadherin-6 mRNA generates additional isoforms with deletions in the extracellular region or a substituted and truncated cytoplasmic domain (4). It interacts homotypically as well as heterotypically with Cadherin-9 and more weakly with Cadherins-7, -10, and -14 (5, 6). Cadherin-6 is expressed primarily in the kidney and brain, both during development and in the adult (3, 7-9). It is enriched in renal proximal tubule epithelial cells and their precursors at sites of cell-cell contact as well as on basolateral membranes (8, 10, 11). In the nervous system, Cadherin-6 is neuronally expressed in discrete regions of the neural plate, neural crest cells that give rise to peripheral nerves, and the brain (7, 12). Cadherin-6 is additionally expressed in the developing retina, osteoclasts and their progenitors, ovarian epithelium, and endometrium during the follicular phase of the menstrual cycle (4, 13-15). It is also expressed in carcinomas of the kidney, ovary, and liver (3, 10, 11, 14).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  3. Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
  4. Mbalaviele, G.  et al. (1998) J. Cell Biol.  141:1467.
  5. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  6. Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.
  7. Inoue, T. et al. (1998) Dev. Dyn. 211:338.
  8. Xiang Y.-Y. et al. (1994) Cancer Res. 54:3034.
  9. Cho, E.A. et al. (1998) Development 125:803.
  10. Paul, R. et al. (1997) Cancer Res. 57:2741.
  11. Shimazui, T. et al. (2000) Eur. Urol. 38:331.
  12. Inoue, T. et al. (1997) Dev. Biol. 183:183.
  13. Ruan, G. et al. (2006) Mech. Dev. 123:881.
  14. Sellar, G.C. et al. (2001) Cancer Res.  61:6977.
  15. Getsios, S. et al. (1998) Dev. Dyn. 211:238.

Alternate Names

Cadherin6, CDH6, K-Cadherin

Entrez Gene IDs

1004 (Human)

Gene Symbol

CDH6

UniProt

P55285

Additional Cadherin-6/KCAD Products

Product Documents for Recombinant Human Cadherin-6/KCAD Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Cadherin-6/KCAD Fc Chimera Protein, CF

For research use only

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