Recombinant Human CANT1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6720-NT
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Calcium Activated Nucleotidase 1/CANT1 protein
Gly80-Ile401, with an N-terminal 6-His tag
Gly80-Ile401, with an N-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
37 kDa
SDS-PAGE
36-40 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the beta 5'-phosphate group from the substrate uridine-5'-diphosphate (UDP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >85,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >85,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
6720-NT
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Calcium Activated Nucleotidase 1/CANT1
Calcium Activated Nucleotidase 1 (CANT1), is a Ca2+-dependent nucleoside diphosphatase which can hydrolyze a variety of nucleoside di- and triphosphates. CANT1 is also known as SCAN-1 and SHAPY. Its preferred substrates are UDP and GDP, and it is not active against nucleoside monophosphates (1). The CANT1 gene encodes a Type II membrane protein that is anchored in the endoplasmic reticulum (ER) membrane with its catalytic domain in the ER lumen (2). A secreted, soluble form of the enzyme also exists (1). CANT1 is expressed in many tissues, including fibroblasts and chondrocytes. Mutations of CANT1 are a cause of Desbuquois dysplasia, a condition characterized by deformation of bones and joints (2, 3). Recombinant human CANT1 was expressed as a secreted, soluble protein.
References
- Smith, T. et al. (2002) Arch. Biochim. Biophys. 406:105.
- Failer, B.U. et al. (2002) J. Biol. Chem. 277:36978.
- Huber, C. et al. (2009) Am. J. Hum. Genet. 85:706.
- Faden, M. et al. (2010) Am. J. Med. Genet. A. 152A:1157.
Long Name
Soluble Calcium Activated Nucleotidase 1
Alternate Names
DBQD, SCAN1, SHAPY
Gene Symbol
CANT1
UniProt
Additional Calcium Activated Nucleotidase 1/CANT1 Products
Product Documents for Recombinant Human CANT1 Protein, CF
Product Specific Notices for Recombinant Human CANT1 Protein, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd.
For research use only
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