Recombinant Human Carboxypeptidase A2/CPA2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2896-ZN
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Carboxypeptidase A2/CPA2 protein
Leu17-Tyr417, with a C-terminal 10-His tag
Leu17-Tyr417, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu17
Predicted Molecular Mass
46 kDa
SDS-PAGE
43 kDa, reducing conditions
Activity
Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2896-ZN
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 200 μg/mL in 25 mM Tris and 150 mM NaCl, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Carboxypeptidase A2/CPA2
Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (residues 1 to 16), a pro region (residues 17 to 112), and a mature chain (residues 113 to 417). The purified rhCPA2 corresponds to the pro form, which can be activated and assayed under the conditions described in the Activity Assay Protocol.
References
- Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.
Alternate Names
CPA2
Entrez Gene IDs
1358 (Human)
Gene Symbol
CPA2
UniProt
Additional Carboxypeptidase A2/CPA2 Products
Product Documents for Recombinant Human Carboxypeptidase A2/CPA2 Protein, CF
Product Specific Notices for Recombinant Human Carboxypeptidase A2/CPA2 Protein, CF
For research use only
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