Recombinant Human Carnosine Dipeptidase 1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2489-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Carnosine Dipeptidase 1/CNDP1 protein
Pro28-His507, with a C-terminal 10-His tag
Pro28-His507, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Pro28
Predicted Molecular Mass
55 kDa
SDS-PAGE
65 kDa, reducing conditions
Activity
Measured by its ability to cleave carnosine ( beta-Ala-L-His) in a two-step assay.
The specific activity is >250 pmol/min/µg, as measured under the described conditions.
The specific activity is >250 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2489-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Carnosine Dipeptidase 1/CNDP1
The human CNDP1 gene encodes carnosine dipeptidase 1, a member of the M20 family of metalloproteases (1, 2). Also known as X-His dipeptidase, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), CNDP1 is a secreted dipeptidase with a narrow specificity for Xaa-His dipeptides including those with Xaa = beta-Ala (carnosine) and Xaa = gamma-aminobutyric acid (homocarnosine), two naturally occurring dipeptides with potential neuroprotective and neurotransmitter fucntions in the brain. In comparison, a closely related protein known as CNDP2, CPGL or CN2, is a cytosolic nonspecific dipeptidase.
References
- Teufel, M. et al. (2004) J. Biol. Chem. 278:6521.
- Bauer, K. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1022, Academic Press, San Diego.
Alternate Names
Carnosinase 1, CNDP1, CPGL2
Entrez Gene IDs
84735 (Human)
Gene Symbol
CNDP1
UniProt
Additional Carnosine Dipeptidase 1/CNDP1 Products
Product Documents for Recombinant Human Carnosine Dipeptidase 1 Protein, CF
Product Specific Notices for Recombinant Human Carnosine Dipeptidase 1 Protein, CF
For research use only
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