Recombinant Human Caspase-3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 707-C3/CF
Key Product Details
Product Specifications
Source
E. coli-derived human Caspase-3 protein
Ser29-Asp175 (subunit 1) & Ala183-His277 (Asp190Glu) (subunit 2)
Ser29-Asp175 (subunit 1) & Ala183-His277 (Asp190Glu) (subunit 2)
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser29 (subunit 1) & Ala183 (subunit 2)
Predicted Molecular Mass
17 kDa (subunit 1), 11 kDa (subunit 2)
SDS-PAGE
18 kDa and 10 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC.
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
707-C3/CF
Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Sucrose. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Caspase-3
References
-
Chowdhury, I. et al. (2008) Comp. Biochem. Physiol. B 151:10.
-
Boatright, K.M. & G.S. Salvesen (2003) Curr. Opin. Cell Biol. 15:725.
-
Launay, S. et al. (2005) Oncogene 24:5137.
-
Walsh, J.G. et al. (2008) Proc. Natl. Scad. Sci. USA 105:12815.
-
Nicholson, D.W. et al. (1995) Nature 376:37.
-
Tewari, M. et al. (1995) Cell 81:801.
-
Fernandes-Alnemri, T. et al. (1994) J. Biol. Chem. 269:30761.
-
Milisav, I. et al. (2009) Apoptosis 14:1070.
-
Han, Z. et al. (1997) J. Biol. Chem. 272:13432.
-
Rossig, L. et al. (1999) J. Biol. Chem. 274:6823.
-
Rank, K.B. et al. (2001) Protein Expr. Purif. 22:258.
-
Atkinson, E.A. et al. (1998) J. Biol. Chem. 273:21261.
- Cohen, G.M. (1997) Biochem. J. 326:1.
Alternate Names
Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA
Gene Symbol
CASP3
UniProt
Additional Caspase-3 Products
Product Documents for Recombinant Human Caspase-3 Protein, CF
Product Specific Notices for Recombinant Human Caspase-3 Protein, CF
For research use only
Loading...
Loading...
Loading...