Recombinant Human Cathepsin V Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1080-CY
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cathepsin V protein
Val18-Val334, with a C-terminal 10-His tag
Val18-Val334, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val18
Predicted Molecular Mass
37 kDa
SDS-PAGE
40 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1080-CY
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin V
Cathepsin V (also known as Cathepsin L2 and U) is a cysteine protease of the papain family (1). It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0 (2). Cathepsin V shows high amino acid identity with Cathepsin L (77% for the proenzymes and 80% for the mature enzymes) (3). However, Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium whereas Cathepsin L is expressed throughout the body.
References
- Turk, V. et al. (2001) EMBO J. 20:4629.
- Bromme, D. et al. (1999) Biochemistry 38:2377.
- Somoza, J.R. et al. (2000) Biochemistry 39:12543.
Alternate Names
Cathepsin L2, Cathepsin U, CTSL2
Entrez Gene IDs
1515 (Human)
Gene Symbol
CTSV
UniProt
Additional Cathepsin V Products
Product Documents for Recombinant Human Cathepsin V Protein, CF
Product Specific Notices for Recombinant Human Cathepsin V Protein, CF
For research use only
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