Recombinant Human Cathepsin X/Z/P Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 934-CY
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cathepsin X/Z/P protein
Ala21-Val303 (Gly23Val & Ser48Thr), with a C-terminal 10-His tag
Ala21-Val303 (Gly23Val & Ser48Thr), with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala21
Predicted Molecular Mass
33 kDa
SDS-PAGE
41 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >800 pmol/min/µg, as measured under the described conditions.
The specific activity is >800 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
934-CY
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin X/Z/P
Cathepsin X (also known as Cathepsin Z and P) is a cysteine protease of the papain family (1-5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7-10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).
References
- Nagler, D.K. and R. Menard (1998) FEBS Lett. 434:135.
- Santamaria, I. et al. (1998) J. Biol. Chem. 273:16816.
- Deussing, J. et al. (2000) Biochim. Biophys. Acta 1491:93.
- Pungercar, J. and G. Ivanovski (2000) Pflugers Arch. Eur. J. Physiol. 439:R116.
- Pungercar, J. et al. (2000) Pflugers Arch. Eur. J. Physiol. 439:R119.
- Sivaraman, J. et al. (2000) J. Mol. Biol. 295:935.
- Menard, R. et al. (2001) Biol. Chem. 382:839.
- Therrien, C. et al. (2001) Biochemistry 40:2702.
- Klemencic, I. et al. (2000) Eur. J. Biochem. 267:5404.
- Guncar, G. et al. (2000) Structure Fold Des. 8:305.
- Lustigman, S. et al. (1996) J. Biol. Chem. 271:30181.
Alternate Names
Cathepsin P, Cathepsin Z, CTSZ
Gene Symbol
CTSZ
UniProt
Additional Cathepsin X/Z/P Products
Product Documents for Recombinant Human Cathepsin X/Z/P Protein, CF
Product Specific Notices for Recombinant Human Cathepsin X/Z/P Protein, CF
For research use only
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