Recombinant Human CCL1/I-309 Protein
R&D Systems, part of Bio-Techne | Catalog # 272-I
Key Product Details
Product Specifications
Source
E. coli-derived human CCL1/I-309/TCA-3 protein
Lys24-Lys96
Lys24-Lys96
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys24
Predicted Molecular Mass
8.5 kDa
Activity
Measured by its ability to chemoattract BW5147 mouse T lymphoma cells.
The ED50 for this effect is 3-9 ng/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR8.
The ED50 for this effect is 1.5-7.5 ng/mL.
The ED50 for this effect is 3-9 ng/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR8.
The ED50 for this effect is 1.5-7.5 ng/mL.
Reviewed Applications
Read 2 reviews rated 4.5 using 272-I in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 272-I
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 272-I/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL1/I-309/TCA-3
Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma/ delta T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.
CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15 - 16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.
References
- Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci USA 89:2950.
- Miller, M.D. et al. (1990) J. Immunol. 145:2737.
Alternate Names
I-309, P500, SCYA1, SCYA2, TCA-3, TCA3
Gene Symbol
CCL1
UniProt
Additional CCL1/I-309/TCA-3 Products
Product Documents for Recombinant Human CCL1/I-309 Protein
Product Specific Notices for Recombinant Human CCL1/I-309 Protein
For research use only
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