Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera) Protein
R&D Systems, part of Bio-Techne | Catalog # 966-6C
Key Product Details
Source
NS0
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human CCL21/6Ckine protein
| Human 6Ckine (Ser24 - Pro134) (Pro41 Ser) Accession # NP_002980 |
GS | Human Fractalkine mucin-like stalk (Phe103 - Gln341) Accession # P78423 |
KQN | 6-His tag |
| N-terminus | C-terminus | |||
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser24
Predicted Molecular Mass
38.3 kDa
SDS-PAGE
95-105 kDa, reducing conditions
Activity
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL).
The ED50 for this effect is 0.5-1.5 µg/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7.
The ED50 for this effect is 25-100 ng/mL.
The ED50 for this effect is 0.5-1.5 µg/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7.
The ED50 for this effect is 25-100 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 966-6C
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 966-6C/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL21/6Ckine
Fractalkine (also known as neurotactin and CX3CL1) is one of two known transmembrane chemokines and is the only member of the CX3C chemokine subfamily. Fractalkine is a type I membrane protein containing a chemokine domain tethered on a long mucin-like stalk that is connected to a transmembrane domain and a C-terminal intracellular domain. Expression of Fractalkine has been detected on endothelial cells, neurons, dendritic cells and a subset of mononuclear phagocytes. The expression of Fractalkine is upregulated in response to inflammatory signals. Fractalkine binds with high affinity to CX3CR1 and mediates the tight binding of leukocytes expressing CX3CR1 independently of G protein activation under flow conditions. In the 6Ckine/mucin-like stalk chimera, the chemokine domain of human Fractalkine is replaced by human 6Ckine (CCL21), a ligand for CCR7. On a molar basis, soluble 6Ckine and 6Ckine/mucin-like stalk chimera are essentially equipotent in their ability to chemoattract cultured human lymphocytes.
References
- Haskell, C.A. et al. (2000) J. Biol. Chem. 275:34183.
Alternate Names
6Ckine, exodus-2, SCYA21, SLC, TCA-4
Gene Symbol
CCL21
Additional CCL21/6Ckine Products
Product Documents for Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera) Protein
Product Specific Notices for Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera) Protein
For research use only
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